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CRISPRa (SAM) HepG2 Cell Line

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  • BPS Bioscience已认证
  • 78194
  • 美国
  • 2025年12月16日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      1

    • 供应商

      BPS Bioscience

    • 细胞类型

      稳转细胞系

    • 品系

      HepG2

    • 组织来源

      /

    • 相关疾病

      多种

    • 物种来源

      human

    • 免疫类型

      多种

    • 器官来源

    • 运输方式

      干冰

    • 年限

      10

    • 生长状态

      良好

    • 英文名

      CRISPRa (SAM) HepG2 Cell Line

    • 规格

      2管

    This cell line has been engineered for use with the CRISPR Synergistic Activation Mediator (SAM) system to induce transcriptional activation and expression of any gene of interest. Cells stably express a mutated dCas9 (Streptococcus pyogenes CRISPR associated protein 9), lacking any endonuclease activity, fused to VP64, a transcriptional activator. Stable dCas9-VP64 expression is maintained with Blasticidin resistance. Cells also stably express P65 (Transcription Factor p65, or Nuclear Factor NF-κB p65) and HSF1 (Heat Shock Factor 1) fused with an MS2 tag, which is maintained with Hygromycin resistance. When these cells are transfected with an MS2- tagged sgRNA targeting the promoter region of the gene of interest, dCas9-VP64 and MS2-P65-HSF1 are recruited to the site in the genomic DNA and begin transcription, inducing expression of the desired gene.

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    图标文献和实验
    相关实验
    • Development and Properties of HepG2 Cells That Constitutively Express CYP2E1

      2E1 plays an important role in ethanol-induced oxidant stress and ethanol toxicity. To study the biochemical and toxicological properties of CYP2E1, our laboratory developed a HepG2 cell line which constitutively expresses the human CYP2E1 form. These cells

    • Multiplexing Cell Viability Assays

      and thus behind the scope of this chapter.As an illustrating example, the reference compound tamoxifen was used to evaluate its cytotoxic properties using the hepatocellular carcinoma cell line HepG2 cells.

    • Sample preparation (preparative gels)

      of bromophenol blue. The whole final diluted CSF sample was used for in-gel sample rehydration. ELC (erythroleukemia cell line): A monolayer culture of human ELC was grown, rinsed trypsinized and washed as explained in the HEPG2 sample preparation

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