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- BPS Bioscience
- 78149
- 美国
- 2025年12月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
BPS Bioscience
- 细胞类型：
稳转细胞系
- 品系：
CHO
- 组织来源：
/
- 相关疾病：
多种
- 物种来源：
hamster
- 免疫类型：
多种
- 器官来源：
无
- 运输方式：
干冰
- 年限：
10
- 生长状态：
良好
- 英文名：
CD38 / CD19 / Firefly Luciferase CHO Recombinant Cell Line
- 规格：
2管
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文献和实验2. Useful as CD38- and/or CD19-expressing target cells in co-culture assay with CD38- and/or CD19-CAR-T cells, for both CD38/CD19-specific cell killing assay and cytokine production assay.
cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Passages: 17 Karyotype: Hypodiploid, modal No 20Hazard: CYA subclone of the parental CHO cell line, which was derived from the ovary of an adult Chinese hamster. Cells require proline
A general protocol for staining cell for cytometry analysis
) 4.Cells stained with PI and SAv-FITC (no Annexin V-Biotin) Other Staining Controls: A cell line that can be easily induced to undergo apoptosis should be used to obtain positive control staining. It is important to note that the basal level
A general protocol for staining cell for cytometry analysis
according to cell type, and stage of apoptosis. In some cases, investigators may also need to reduce the number of cells to 0.5 x 10 5 /100 µl and still add 5-15 µg of recombinant Annexin V to obtain optimal results. 4. Gently mix the cells
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