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- BPS Bioscience
- 60410
- 美国
- 2025年12月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
1
- 供应商：
BPS Bioscience
- 细胞类型：
稳转细胞系
- 品系：
HEK293
- 组织来源：
/
- 相关疾病：
多种
- 物种来源：
human
- 免疫类型：
多种
- 器官来源：
无
- 运输方式：
干冰
- 年限：
10
- 生长状态：
良好
- 英文名：
PDE10A-HEK293 Recombinant Cell line
- 规格：
2管
N-terminal His-tagged human PDE10A2 has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE10A2 expression was confirmed by Western blotting. The function of PDE10A2 was confirmed by cAMP detection assay. Forskolin is commonly used to raise levels of intracellular cyclic AMP (cAMP). When HEK293 cells are activated by forskolin, cAMP levels are upregulated in parental cells, whereas cells overexpressing PDE10A show reduction in cAMP accumulation. Inhibition of PDE10A activity restores cAMP levels.
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文献和实验指从某初代培养开始通过连续培养所产生的所有细胞。包括从初代培养的细胞和细胞群所发展的一系列的细胞。如果培养的状态清楚，则在前面冠以有限继代性（ finite）或连续继代性（ continuous）。例如，人二倍体成纤细胞系属于前者， HeLa细胞系属后者。另外连续继代性细胞系也称为树立细胞系。
Cell Assessment by At-line Microscopy
and at-line cell monitoring in bioreactors (Akin et al., Biosens Bioelectron 26:4532–4537, 2011; Babitzky et al., At-line microscopic analysis of suspension cell cultures. In: ECCE/ECAB, the first joint European Congress of chemical engineering and applied
A method is described to establish mouse embryonic stem cell (ESC) lines from hybrid and inbred strains of mice. Attention is paid not only to the methodology for isolation and culture but also to the validation of freshly derived lines, in order
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