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| BioReady Taq Mix (with dye) | 博日 | BSA31M1B | 450 | 5ml | 科研 | 盒 | 248 | 180 | 144 | 135 | / | 50,100盒 |
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文献和实验| BioReady Taq Mix (with dye) | 博日 | BSA31M1B | 450 | 5ml | 科研 | 盒 | 248 | 180 | 144 | 135 | / | 50,100盒 |
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye primers
mix 30/60ul diluted Taq polymerase 30/60ul respective fluorescent end-labeled primer 120/240ul [1] [2] 下一页
Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions
in the reaction mix. We have recently observed that the nested fragment set distribution for the DNA cycle sequencing reactions using the fluorescent labelled terminators (8) is much less sensitive to DNA concentration than that obtained with the fluorescent
, 1.6 µl 4dNTP mix (25 µM), 2 µl T12 MN primer, 2 µl cDNA, 0.2 µl Taq DNA polymerase (5 U/µl), 1 µl [a- 33P]dCTP aliquot and add 2 µl arbitrary primer (2 pmol/µl) run PCR: 40 cycles (94 °C, 30 sec.) (40 °C, 2 min.) (72 °C, 30 sec.), 1 cycle
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