- 询价
- BioLegend
- 902901
- BioLegend
- 2025年11月15日
5 年金牌会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
BioLegend 902901 Purified anti-Nuclear Pore Complex Proteins
- 供应商：
北京云肽生物科技有限公司
BioLegend 902901 Purified anti-Nuclear Pore Complex Proteins
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
美国 BioLegend 公司位于美国加州，致力于为生物医药研究的各个前沿领域提供高质量的抗体及其它相关试剂。BioLegend 虽然成立的时间不长，但它聚集了一批来自 PharMingen 公司的专业人士，建立了一支具有雄厚技术背景的专业队伍。通过其自身的先天优势，以及与世界知名实验室和研究所的良好合作关系，以合理的价格为广大客户提供高质量的免疫学和细胞生物学产品。
BioLegend 公司以其专业技术力量为保证，提供各种质量卓越的产品，并不断地开发新产品，以满足飞速发展的生命科学研究的需要。
BioLegend 902901 Purified anti-Nuclear Pore Complex Proteins
产品简介：
产品品牌：BioLegend
产品货号：902901
产品名称：BioLegend 902901 Purified anti-Nuclear Pore Complex Proteins
产品规格：100 µL
产品说明：
Clone MAb414 (See other available formats)
Regulatory Status RUO
Other Names
107 kD nucleoporin, NPC proteins, Nuclear pore complex protein Nup107, Nucleoporin 107kD, Nucleoporin Nup107, NUP 107, NUP 84, NUP107, NUP153, NUP155, NUP84, NUP98
Previously Covance Catalog# MMS-120P
Isotype Mouse IgG1
描述
核孔是穿过核膜的大型蛋白质复合体。构成核孔复合体的蛋白质被称为核通道蛋白。大约一半的核通道蛋白通常含有螺线管蛋白结构域——要么是α螺线管，要么是β螺旋折叠，或者在某些情况下两者都是独立的结构域。每个NPC包含至少456个单独的蛋白质分子，由30种不同的蛋白质（核通道蛋白）组成。另一半表现出“天然未折叠”或内在无序蛋白质的典型结构特征，即它们是缺乏有序二级结构的高度柔性蛋白质。这些紊乱的蛋白质是FG核通道蛋白，之所以这么叫，是因为它们的氨基酸序列含有许多苯丙氨酸-甘氨酸重复序列。
核孔复合物允许分子穿过核膜传输。这种转运包括从细胞核转移到细胞质的RNA和核糖体蛋白，以及进入细胞核的蛋白质（如DNA聚合酶和层粘连蛋白）、碳水化合物、信号分子和脂质。尽管较小的分子只是通过孔扩散，但较大的分子可能会被特定的信号序列识别，然后在核通道蛋白的帮助下扩散到细胞核中或从细胞核中扩散出来。。围绕实际孔隙（外圈）的八个蛋白质亚基中的每一个都在孔道上投射出一个辐条状蛋白质。
核孔蛋白p62（p62）蛋白仍然与核孔复合体纤层部分相关。p62被合成为61kDa的可溶性细胞质前体，随后进行修饰，包括添加N-乙酰葡糖胺残基，然后与其他复杂蛋白质结合。该基因编码的蛋白质是含有核孔蛋白的FG重复序列的成员，定位于核孔中心塞。该蛋白质与导入蛋白α/β复合物结合，该复合物参与含有核定位信号的蛋白质的导入。该基因的多个转录物变体编码单一的蛋白质异构体。
P62是一种富含丝氨酸/苏氨酸的蛋白质，约520个氨基酸，氨基末端有四肽重复序列，还有一系列具有疏水性七肽重复序列的α螺旋区。P62组装成一个包含3个附加蛋白的复合体，即p60、p54和p45，形成约235kDa的P62复合体。糖基化似乎参与p62组装和分解成更高阶复合物，Ser270至Thr294之间富含丝氨酸/苏氨酸的连接区似乎是调节性的。p62复合物定位于孔复合物的核质侧和细胞质侧，p62复合体相对于核孔复合体的相对直径表明它在孔门控中相互作用。
p62复合物的抗体参与1种或多种自身免疫性疾病。P62糖基化在糖尿病中增加。p62在IV期原发性胆汁性肝硬化中也更常见，并且是严重疾病的预后。p62产生的减少与阿尔茨海默病有关。人们认为p62启动子的氧化损伤与AD和其他神经退行性疾病有关。
Nuclear pores are large protein complexes that cross the nuclear envelope. The proteins that make up the nuclear pore complex are known as nucleoporins. About half of the nucleoporins typically contain solenoid protein domains—either an alpha solenoid or a beta-propeller fold, or in some cases both as separate structural domains. Each NPC contains at least 456 individual protein molecules and is composed of 30 distinct proteins (nucleoporins). The other half show structural characteristics typical of "natively unfolded" or intrinsically disordered proteins, i.e. they are highly flexible proteins that lack ordered secondary structure. These disordered proteins are the FG nucleoporins, so called because their amino-acid sequence contains many phenylalanine—glycine repeats.
Nuclear pore complexes allow the transport of molecules across the nuclear envelope. This transport includes RNA and ribosomal proteins moving from nucleus to the cytoplasm and proteins (such as DNA polymerase and lamins), carbohydrates, signaling molecules and lipids moving into the nucleus. Although smaller molecules simply diffuse through the pores, larger molecules may be recognized by specific signal sequences and then be diffused with the help of nucleoporins into or out of the nucleus.. Each of the eight protein subunits surrounding the actual pore (the outer ring) projects a spoke-shaped protein over the pore channel.
Nucleoporin p62 (p62) protein remains associated with the nuclear pore complex-lamina fraction. p62 is synthesized as a soluble cytoplasmic precursor of 61 kDa followed by modification that involve addition of N-acetylglucosamine residues, followed by association with other complex proteins.The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform.
P62 is a serine/threonine rich protein of ~520 amino acids, with tetrapeptide repeats on the amino terminus and a series of alpha-helical regions with hydrophobic heptad repeats. P62 assembles into a complex containing 3 addition proteins, p60, p54 and p45 forming the p62 complex of ~235 kDa. Glycosylation appears to be involved in the assembly and disassembly of p62 into higher order complexes, and a serine/threonine rich linker region between Ser270 to Thr294 appear to be regulatory. The p62 complex is localized to both the nucleoplasmic and cytoplasmic sides of the pore complex and the relative diameter of p62 complex relative to the nuclear pore complex suggests it interacts in pore gating.
Antibodies to p62 complex are involved in 1 or more autoimmune diseases. P62 glycosylation is increased in diabetes. p62 is also more frequent in Stage IV primary biliary cirrhosis and is prognostic for severe disease. Reduced p62 production has been linked to Alzheimer's disease. It is thought oxidative damage of the p62 promoter is correlated with AD and other neurodegenerative disorders.
Product Details
Isotype Control Purified Mouse IgG1, κ Isotype Ctrl
Verified Reactivity Vertebrate, Xenopus, Yeast
Antibody Type Monoclonal
Host Species Mouse
Immunogen
The antibody was raised using a nuclear pore complex mixture.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration 1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application ICC - Quality tested
WB, IHC-P - Verified
IHC-F, IP, IEM - Reported in the literature, not verified in house
Recommended Usage
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.5 - 1 μg/mL (1:1000 - 1:2000 dilution) is recommended. For Western blotting, the suggested use of this reagent is 0.2 - 0.5 µg per mL (1:2000 - 1:5000). For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 1 µg/mL is suggested. For immunohistochemical staining on frozen tissue sections, a dilution of 1:500 is suggested. For immunoprecipitation and electron microscopy, the suggested dilution of this reagent is 1:5000. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family Nuclear Markers
以下是 BioLegend 品牌部分产品，如需其他产品请联系本公司销售。
BioLegend 104537 Brilliant Violet 711™ anti-mouse CD69 H1.2F3 50 ug
BioLegend 104541 Brilliant Violet 650™ anti-mouse CD69 H1.2F3 50 ug
BioLegend 104543 Brilliant Violet 785™ anti-mouse CD69 H1.2F3 50 ug
BioLegend 104545 Brilliant Violet 421™ anti-mouse CD69 H1.2F3 50 ug
BioLegend 104548 APC/Fire™ 750 anti-mouse CD69 H1.2F3 25 ug
BioLegend 104549 APC/Fire™ 750 anti-mouse CD69 H1.2F3 100 ug
BioLegend 104553 KIRAVIA Blue 520™ anti-mouse CD69 H1.2F3 25 ug
BioLegend 104605 PE anti-mouse CD70 FR70 50 ug
BioLegend 104606 PE anti-mouse CD70 FR70 200 ug
BioLegend 104702 Purified anti-mouse CD80 16-10A1 500 ug
BioLegend 104706 FITC anti-mouse CD80 16-10A1 500 ug
BioLegend 104707 PE anti-mouse CD80 16-10A1 50 ug
BioLegend 104708 PE anti-mouse CD80 16-10A1 200 ug
BioLegend 104711 PE/Cyanine5 anti-mouse CD80 16-10A1 25 ug
BioLegend 104712 PE/Cyanine5 anti-mouse CD80 16-10A1 100 ug
BioLegend 104713 APC anti-mouse CD80 16-10A1 25 ug
BioLegend 104714 APC anti-mouse CD80 16-10A1 100 ug
BioLegend 104715 Alexa Fluor® 488 anti-mouse CD80 16-10A1 25 ug
BioLegend 104716 Alexa Fluor® 488 anti-mouse CD80 16-10A1 100 ug
BioLegend 104717 Alexa Fluor® 647 anti-mouse CD80 16-10A1 25 ug
BioLegend 104721 PerCP/Cyanine5.5 anti-mouse CD80 16-10A1 25 ug
BioLegend 104722 PerCP/Cyanine5.5 anti-mouse CD80 16-10A1 100 ug
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
A Functional Proteomic Study of the Trypanosoma brucei Nuclear Pore Complex: An Informatic Strategy
The nuclear pore complex (NPC) is the sole mediator of transport between the nucleus and the cytoplasm. The NPC is composed of about 30 distinct proteins, termed nucleoporins or nups. The yeast (Rout et al., J Cell Biol 148:635–651, 2000
In bacteria, proteins are secreted across the cytoplasmic membrane by a protein complex termed translocase. The ability to study the activity of the translocase in vitro using purified proteins has been instrumental for our understanding
Reconstitution of Nuclear Import in Permeabilized Cells
factors. The latter include receptors (karyopherins) that recognize the cargoes and carry them across the nuclear pore complex (NPC), and the small GTPase Ran, which modulates karyopherin–cargo binding. Nuclear import can be studied in vitro using
技术资料暂无技术资料 索取技术资料
BioLegend 902901 Purified anti-Nuclear Pore Complex Proteins
询价
