BioLegend 791902 Recombinant Human IL-2 (E. coli expressed, carrier-free) 产品简介:BioLegend 产品货号:791902 产品名称:BioLegend 791902 Recombinant Human IL-2 (E. coli expressed, carrier-free) 产品规格:10 µg
产品说明: Regulatory Status RUO Other Names T-cell growth factor (TCGF), Eosinophil differentiation factor (EDF), Killer cell helper factor (KHF), Macrophage-activating factor for cytotoxicity I (MAF-C I), Thymocyte differentiation factor (TDF)
描述 IL-2是通过其作为T细胞生长因子(TCGF)的功能而被发现的。它在对抗病原性感染的免疫反应中起着关键作用。TCRs对外来抗原的识别和结合刺激IL-2的分泌和IL-2Rs在T细胞表面的表达。随后,IL-2/IL-2R相互作用激活细胞内Ras/Raf/MAPK、JAK/STAT和PI3K/AKT信号通路,并最终刺激Ag选择的细胞毒性T细胞的生长、分化和存活。人IL-2作用于小鼠和人T细胞,其受体由其他细胞因子共享。IL-2Rα是一种IL-2特异性受体,IL-2Rβ与IL-15和γ链共有,γ链是许多细胞因子共有的受体,包括IL-2、IL-4、IL-7、IL-9、IL-15和IL-21。 IL-2 was discovered through its function as a T cell growth factor (TCGF). It plays a pivotal role in immune responses against pathogenic infection. Recognition and binding of foreign antigens by the TCRs stimulate both the secretion of IL-2 and the expression of IL-2Rs on the T cell surface. Subsequently, the IL-2/IL-2R interaction activates the intracellular Ras/Raf/MAPK, JAK/STAT, and PI3K/AKT signaling pathways, and ultimately stimulates the growth, differentiation, and survival of the Ag-selected cytotoxic T cells. Human IL-2 acts on murine and human T cells, and its receptors are shared by other cytokines. IL-2Rα is an IL-2–specific receptor, IL-2Rβ is shared with IL-15 and the γ chain that is a common receptor shared by many cytokines including IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21.
Product Details Source Human IL-2, amino acid (Ala21-Thr153) (Accession: # P60568) was expressed in E. coli.
Molecular Mass The 133 amino acid recombinant protein has a predicted molecular mass of approximately 15.4 kD. The DTT-reduced and non-reduced protein migrates at approximately 15 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
Purity > 95%, as determined by Coomassie stained SDS-PAGE Formulation 0.22 µm filtered protein solution is in 0.1% TFA, 10% Acetonitrile
Endotoxin Level Less than 0.1 EU per µg cytokine as determined by the LAL method
Concentration 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling Unopened vial can be stored between 2°C and 8°C for up to 2 weeks at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity Recombinant human IL-2 induces the proliferation of mouse HT-2 cells in a dose- dependent manner. The ED50 for this effect is 0.3 - 1.5 ng/mL. Application Bioassay
Antigen Details Structure Monomer Distribution T cells (CD4+ and CD8+), gamma δ T cells, B cells, dendritic cells, and eosinophils. Mainly activated T cells.
Function Differentiation of Th1 and Th2 cells, inhibition of Th17 and Tfh cell differentiation, regulation of Treg differentiation, T cell proliferation, B cell proliferation, promotes CD8 and NK cell cytolytic activity.
Interaction CD4 T cells, T reg cells, CD8 effector cells, NK cells, B cells Ligand/Receptor Trimeric receptor: IL-2Rα (CD25), IL-2Rβ (CD122), and γc (CD132) and dimeric receptor:IL-2Rβ (CD122) and γc (CD132)
Bioactivity Recombinant human IL-2 induces the proliferation of mouse HT-2 cells.
Cell Type Embryonic Stem Cells, Hematopoietic stem and progenitors Biology Area Immunology, Stem Cells Molecular Family Cytokine/Chemokine Receptors