BioLegend 781304 Recombinant Human MICB-Fc Chimera (carrier-free) 产品简介: 产品品牌:BioLegend 产品货号:781304 产品名称:BioLegend 781304 Recombinant Human MICB-Fc Chimera (carrier-free) 产品规格:25 µg
产品说明: Regulatory Status RUO Other Names MHC class I polypeptide-related sequence B, PERB11.2, MHC Class I-Like Molecule PERB11.2-IMX, MHC Class I Chain-Related Protein B, Stress Inducible Class I Homolog, MHC Class I Mic-B Antigen, MIC-B
描述 MICB,人类主要组织相容性复合体(MHC)I类多肽相关序列B是MIC家族的成员,包括MICA、MICB和五个假基因MICC至MICG。MICB是一种由383个氨基酸组成的蛋白质,与NKG2D受体和T细胞受体(TCR)相互作用,激活NK细胞、αβCD8+T细胞和γδT细胞。MICA和MICB包含两个Ig样结构域(α1,α2)和C型Ig样α3结构域、一个跨膜结构域和一个羧基末端细胞内尾部。MICA和MICB在大多数哺乳动物中是保守的,但在啮齿类动物中不是。MICA和MICB具有高度相似性(84%相同)。MICA和MICB与MHC I类家族不同,当逐域比较时,它们呈现出28%至35%的相似性。MICA和MICB与NKG2D结合,并且MICB与受体的结合可以被嵌合形式的UL16阻断;表明UL16与NK细胞上NKG2D中存在的结合表位结合或重叠。ADAM15已被鉴定参与PANC-1胰腺癌症细胞中MICB的脱落。血清中的可溶性MICB与多发性硬化症、乙型肝炎病毒感染以及结肠癌、卵巢癌、前列腺癌、乳腺癌、肾癌和黑色素瘤等实体瘤有关。黑色素瘤患者血清中可溶性MICB水平高与预后不良有关。 MICB, human major histocompatibility complex (MHC) class I polypeptide-related sequence B is a member of the MIC family that includes MICA, MICB, and five pseudogenes MICC to MICG. MICB is a protein of 383 amino acids and interacts with the NKG2D receptor and the T cell receptor (TCR) to activate NK cells, αβ CD8+ T cells, and γδ T cells. MICA and MICB contain two Ig-like domains (α1, α2) and the C-type Ig-like α3 domain, a transmembrane domain, and a carboxyl-terminal intracellular tail. MICA and MICB are conserved in most mammals, but not in rodents. MICA and MICB have high similarity (84% identical). MICA and MICB diverge from the MHC class I family, when they are compared domain by domain, they present a similarity of 28 to 35%. MICA and MICB bind to NKG2D, and the binding of MICB to the receptor can be blocked by a chimeric form of UL16; indicating that UL16 binds to the same, or overlaps with the binding epitope present in NKG2D on the NK cells. ADAM15 has been identified to participate in the shedding of MICB in PANC-1 pancreatic cancer cells. Soluble MICB in serum has been associated with multiple sclerosis, hepatitis B viral infection, in addition to solid tumors such as carcinomas of colon, ovary, prostate, breast, kidney, and melanoma. High levels of soluble MICB in serum of melanoma patients are associated to poor prognosis.
Product Details Source Human MICB, amino acids Ala23-Gly298 (Accession# NP_005922) was expressed in 293E cells. Human IgG1-Fc is at the carboxy-terminus.
Molecular Mass The 515 amino acid recombinant protein has a predicted molecular mass of approximately 58.4 kD. The DTT-reduced and non-reduced protein migrate at approximately 70 kD and 140 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Ala.
Purity >95%, as determined by Coomassie stained SDS-PAGE. Formulation 0.22 µm filtered protein solution is in PBS pH7.2. Endotoxin Level Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity Recombinant human MICB binds to recombinant human NKG2D in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 15 – 60 ng/mL. Application Bioassay
Antigen Details Structure Dimer Distribution Gastrointestinal epithelium, stressed airway epithelial cells, fibroblasts, cancer cell lines, cervical cancer, and myelomonocytic leukemia.
Function Modulates immune responses in cancer, viral infections, and autoimmune diseases. MICB expression is regulated by RNA-binding proteins, induced by heat shock, and oxidative stress in airway epithelial cells.
Interaction NK cells, CD8 αβ T cells, and γδ T cells Ligand/Receptor NKG2D, NKG2D/DAP10 complex Bioactivity Recombinant human MICB binds to recombianant NKG2D in a dose-dependent manner using a functional ELISA. Cell Type NK cells, NKT cells Biology Area Innate Immunity Molecular Family Soluble Receptors