BioLegend 769406 Recombinant M

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BioLegend 769406 Recombinant Mouse TRANCE (RANKL, TNFSF11) (carrier-free)
产品简介：
产品品牌：BioLegend
产品货号：769406
产品名称：BioLegend 769406 Recombinant Mouse TRANCE (RANKL, TNFSF11) (carrier-free)
产品规格：100 µg

产品说明：
Regulatory Status   RUO
Other Names
Tumor necrosis factor ligand superfamily, member 11 (TNFSF11), Osteoprotegerin ligand (OPGL), Receptor activator of NF-kappa-B ligand (RANKL), TNF-related activation-induced cytokine (Trance), Osteoclast differentiation factor (ODF), CD254.

描述
RANKL基因编码一种由316个氨基酸组成的II型膜蛋白，预测分子量为35kD。RANKL被切割以产生具有生物活性的可溶性形式。膜结合RANKL的脱落似乎是由MMP14和ADAM10的表达介导的。原代成骨细胞中MMP14的抑制增加了膜结合的RANKL，并促进了与巨噬细胞共培养的破骨细胞生成。因此，RANKL脱落似乎是下调局部破骨细胞生成的一个重要过程。或者，成骨细胞增加RANKL的产生导致破骨细胞分化、活化和存活，从而导致骨吸收增加。RANKL与其受体RANK的结合激活TNF受体相关因子6（TRAF6），该因子与包括NF-κB、c-jun N-末端激酶（JNK）或Src在内的下游途径有关。TRAF6已被证明是通过增强破骨细胞功能所必需的Src激酶来分化破骨细胞所必需的。
RANKL gene encodes a type II membrane protein of 316 amino acids with a predicted molecular mass of 35 kD. RANKL is cleaved to produce a soluble form with biological activity. The shedding of membrane-bound RANKL appears to be mediated by expression of MMP14 and ADAM10. Suppression of MMP14 in primary osteoblasts increases membrane-bound RANKL and promotes osteoclastogenesis in cocultures with macrophages. Therefore, RANKL shedding seems to be an important process that down-regulates local osteoclastogenesis. Alternatively, an increased production of RANKL by osteoblastic cells leads to osteoclast differentiation, activation, and survival, which results in increased bone resorption. Binding of RANKL to its receptor RANK activates TNF receptor-associated factor 6 (TRAF6), which is linked to downstream pathways including NF-κB, c-jun N-terminal kinase (JNK) or Src. TRAF6 has been shown to be necessary for the differentiation of osteoclastic cells by enhancing Src kinase, essential for osteoclast function.

Product Details
Source
Mouse RANKL, amino acid Lys158-Asp316 (Accession # O35235) with an N-terminal Met, was expressed in E. coli.

Molecular Mass
The 160 amino acid recombinant protein has a predicted molecular mass of approximately 17.9 kD. The DTT-reduced and non-reduced protein migrates at approximately 19 kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Met.

Purity
>98%, as determined by Coomassie stained SDS-PAGE.
Formulation
Sterile-filtered with 0.2 µm filter, solution comprised of 20 mM sodium phosphate, pH 6.0, 100 mM NaCl, and 2 mM EDTA.

Endotoxin Level
< 0.1 EU/µg cytokine as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity
The ED50= 0.75-3 ng/ml, as determined by induction of osteoclast differentiation in RAW264.7 mouse macrophage cell line, measured by TRAP reaction.
Application   Bioassay

Antigen Details
Structure   Cytokine
Distribution
Predominantly expressed in monocytes, macrophages, polymorphonuclear cells, B cells. Detected in microglial cells and Treg cells. Inducible in keratinocytes and endothelial cells.

Function
Potent stimulator of osteoclast formation from precursor cells and bone-resorbing activity in mature osteoclasts. Augments the ability of dendritic cells to stimulate naïve T-cell proliferation. Activates JNK and NF-κB and induces the expression of IL-1, IL-6, IL-12, and IL-15 in dendritic cells. Crucial factor in lymphocyte development and lymph node organogenesis. Stimulates proliferation, adhesion, and IL-7 expression of thymic epithelial cells. RANKL expression, in human and murine osteoblastic cells, is stimulated by various cytokines (IL-1, TNF-α and IL-11) and calciotrophic hormones including PTH, 1, 25-dihydroxyvitamin D3 (1,25D3), and prostaglandin E2.

Interaction
T cells, dendritic cells, bone marrow derived macrophages, osteoclast precursors and mature osteoclasts.

Ligand/Receptor
TNFRSF11A (Tumor necrosis factor receptor superfamily, member 11A) also called RANK and TNFRSF11B (OPG) which acts as a decoy receptor for RANKL and as a potent inhibitor of osteoclast formation.
Cell Type   Embryonic Stem Cells
Biology Area   Immunology, Stem Cells
Molecular Family   Growth Factors, Cytokines/Chemokines

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