BioLegend 761904 Recombinant Human Asprosin (carrier-free) 产品简介: 产品品牌:BioLegend 产品货号:761904 产品名称:BioLegend 761904 Recombinant Human Asprosin (carrier-free) 产品规格: 25 µg
产品说明: Regulatory Status RUO Other Names ASPRO, C-terminal cleavage product of Fibrillin 1 (FBN1)
描述 纤维蛋白是细胞外微纤维的主要组成成分,广泛分布在全身的弹性和非弹性结缔组织中。天冬氨酸是原纤维蛋白的C末端裂解产物。天冬氨酸是一种禁食诱导的糖原,通过靶向肝脏释放葡萄糖并提高血糖水平来对低血糖做出反应。它由白色脂肪组织分泌,并在血浆中以纳摩尔水平循环。天冬氨酸通过激活G蛋白cAMP蛋白激酶A途径促进肝细胞中葡萄糖的释放。Asprosin在人类和小鼠胰岛素抵抗的病理学上升高,其水平的降低可以预防代谢综合征相关的高胰岛素血症。 Fibrillin is the major constitutive element of extracellular microfibrils and has widespread distribution in both elastic and non-elastic connective tissue throughout the body. Asprosin is the C-terminal cleavage product of pro-fibrillin. Asprosin is a fasting-induced glucogenic protein that responds to low dietary glucose by targeting the liver to release glucose and increase plasma glucose levels. It is secreted by white adipose tissue and circulates in the plasma at nanomolar levels. Asprosin promotes glucose release in hepatocytes by activating G protein-cAMP-protein kinase A pathway. Asprosin is shown to be pathologically elevated with human and mouse insulin resistance, and the reduction of its level protects against metabolic-syndrome-associated hyperinsulinism.
Product Details Source Human Asprosin, amino acids Ser2732-His2871 (Accession# P35555) was expressed in 293E cells.
Molecular Mass The 140 amino acids recombinant protein has a predicted molecular mass of approximately 16 kD. The DTT-reduced and non-reduced proteins migrate at approximately 30 kD by SDS-PAGE. The predicted N-terminal amino acid is Ser.
Purity >95% as determined by Coomassie stained SDS-PAGE. Formulation 0.22 µm filtered protein solution is in 10mM NaH2PO4, 0.3M NaCl, pH 7.2.
Endotoxin Level Less than 1 EU per µg protein as determined by the LAL method. Concentration 10 and 25 µg sizes are bottled at 200 µg/mL.
Storage & Handling Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. Recommended Usage Bioassay
Antigen Details Distribution Secreted by white adipose tissue. Function Promotes glucose release in hepatocytes. Interaction Hepatocytes. Molecular Family Growth Factors, Cytokines/Chemokines
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