产品说明: Regulatory Status RUO Other Names CD274, Programmed cell death 1 ligand 1 (PD-L1), B7 homolog 1 (B7h1)
描述 PD-L1是一种由290个氨基酸组成的I型跨膜蛋白,是B7家族的成员。小鼠PD-L1与其人类同源物具有70%的氨基酸同一性。PD-L1与其受体PD-1的结合导致T细胞受体介导的淋巴细胞增殖和细胞因子分泌的抑制。PD-L1在用低水平的抗CD3刺激的T细胞中诱导IL-10的产生。PD-L1/PD-1相互作用抑制针对自身抗原和肿瘤的免疫反应,并在维持外周免疫耐受中发挥重要作用。PD-L1基因的破坏导致T细胞反应的上调和自身反应性T细胞的产生。抗PD-1或PD-L1的抗体可提高抗肿瘤免疫力。PDL1在异基因妊娠模型中赋予胎儿耐受性方面具有重要作用;针对PD-L1的抗体导致母体对胎儿的耐受性崩溃。PD-L1与PD-L2(CD273、B7-DC)共享其受体。PD-L2的表达比PD-L1更有限,在活化的巨噬细胞和树突状细胞上表达。PD-L1在许多肿瘤中表达,与其受体的相互作用激活了抑制T细胞活性的信号通路,从而抑制了抗肿瘤免疫反应。靶向PD1或PD-L1的抗体阻断PD1通路并重新激活T细胞活性。 PD-L1 is a type I transmembrane protein of 290 amino acids, and it is a member of the B7 family. Mouse PD-L1 has 70% amino acid identity to its human orthologue. Binding of PD-L1 to its receptor PD-1 leads to the inhibition of T cell receptor–mediated lymphocyte proliferation and cytokine secretion. PD-L1 induces IL-10 production in T cells stimulated with low levels of anti-CD3. PD-L1/PD-1 interaction suppresses immune responses against autoantigens and tumors and plays an important role in the maintenance of peripheral immune tolerance. Disruption of the PD-L1 gene leads to up-regulated T cell responses and the generation of self-reactive T cells. Antibodies against PD-1 or PD-L1 leads to increased antitumor immunity. PDL1 has an important role in conferring fetomaternal tolerance in an allogeneic pregnancy model; antibodies against PD-L1 lead to a breakdown in maternal tolerance to the fetus. PD-L1 shares its receptor with PD-L2 (CD273, B7-DC). PD-L2 has a more limited expression than PD-L1, being expressed on activated macrophages and dendritic cells. PD-L1 is expressed in many tumors, and the interaction with its receptor activates signaling pathways that inhibit T-cell activity and therefore the antitumor immune response. Antibodies targeting PD1 or PD-L1 block the PD1 pathway and reactivate T cell activity.
Product Details Source Mouse B7-H1, amino acids (Phe19-Thr238) (Accession# NM_021893), was expressed in 293E cells. Human IgG-Fc is in the carboxy-terminus.
Molecular Mass The 458 amino acid recombinant protein has a predicted molecular mass of approximately 51.5kD. The DTT-reduced and non-reduced protein migrate at approximately 70 - 80kD and 140kD respectively by SDS-PAGE. The predicted N-terminal amino acid is Phe.
Purity >95%, as determined by Coomassie stained SDS-PAGE. Formulation 0.22 µm filtered protein solution is in PBS, pH 7.2.
Endotoxin Level Less than 0.1 EU per µg of protein as determined by the LAL method.
Concentration 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity Immobilized mouse PD-L1 binds mouse PD-1 in a dose dependent manner. The ED50 = 0.1 - 0.5 µg/ml. Recommended Usage Bioassay
Antigen Details Structure Dimer. Distribution APC, monocytes, dendritic cells, expressed in nonlymphoid tissues, and stromal cell.
Function Enhances CD28-independent T-helper cell function, suppresses immune responses against autoantigens, and participates in fetomaternal tolerance.
Interaction Antigen-stimulated T and B cells, regulatory T cells, follicular T and B cells, dendritic cells, and monocytes. Ligand/Receptor PD-1 (CD279). Bioactivity Immobilized mouse PD-L1 binds to its receptor PD-1.
Cell Type Embryonic Stem Cells Biology Area Immunology, Cancer Biomarkers, Costimulatory Molecules, Stem Cells
Molecular Family Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors, Soluble Receptors