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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
齐源
- 库存:
999
- 靶点:
I-A (Aβ ) Antibody
- 级别:
抗小鼠 IA k (Aβ k )荧光标记 FITC 流式抗体
- 目录编号:
抗小鼠 IA k (Aβ k )荧光标记 FITC 流式抗体
- 克隆性:
单克隆
- 抗原来源:
小鼠
- 保质期:
抗小鼠 IA k (Aβ k )荧光标记 FITC 流式抗体
- 抗体英文名:
FITC anti-mouse I-Ak (Aβk) Antibody
- 抗体名:
I-A (Aβ ) Antibody
- 标记物:
FITC
- 宿主:
小鼠
- 适应物种:
小鼠
- 免疫原:
C3H小鼠脾细胞
- 亚型:
抗小鼠 IA k (Aβ k )荧光标记 FITC 流式抗体
- 形态:
抗小鼠 IA k (Aβ k )荧光标记 FITC 流式抗体
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.5毫克/毫升
- 规格:
50 ug
Clone:10-3.6 (See other available formats)
Regulatory Status:RUO
Other Names:MHC class II
Isotype:Mouse (CWB) IgG2a, κ
Ave. Rating:Submit a Review
Product Citations:5 publications
The 10-3.6 antibody reacts with the β chain of the I-Ak MHC class II alloantigen. This class II molecule is expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2k bearing mice and involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 10-3.6 antibody cross-reacts with I-Af,r,s antigens and I-Ag7 of NOD mice; it does not react with other haplotypes (e.g., b, d, p, q).
Isotype Control:FITC Mouse IgG2a, κ Isotype Ctrl (FC)
Verified Reactivity:Mouse
Antibody Type:Monoclonal
Host Species:Mouse
Immunogen:C3H mouse splenocytes
Formulation:Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation:The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration:0.5 mg/ml
Storage & Handling:The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes:
Additional reported applications (for the relevant formats) include immunoprecipitation1,2, protection against autoimmune IDDM3, in vitro blocking of antigen-specific MHC-restricted responses and in vivo inhibition of lymphoma growth4, and immunohistochemical staining5 of a、cetone-fixed frozen sections.
This clone does not cross react with other haplotypes (e.g. b, d, p, q).
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文献和实验(PubMed link indicates BioLegend citation)
1、Landais D, et al. 1986. J. Immunol. 137:3002. (IP)
2、Kupinski JM, et al. 1983. J. Immunol. 130:2277. (IP)
3、Kappler JW, et al. 1981. J. Exp. Med. 153:1198.
4、Alisauskas RM, et al. 1986. Immunopharmacology 12:1.
5、Reis e Sousa and Germain 1999. J. Immunol. 162:6652. (IHC)
6、Yui MA, et al. 2010. J. Immunol. 185:284. PubMed
7、Gaudreau S, et al. 2007. J. Immunol. 179:3638. (FC)
8、Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
Product Citations:
1、Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed
2、Bi CS, et al. 2020. Cell Prolif. 53:e12827. PubMed
3、Lai JH, et al. 2021. iScience. 24(6):102498. PubMed
4、Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
5、Liu WL, et al. 2019. Nat Commun. 10:3199. PubMed
抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
光基团作为抗体和其他生物分子的标签或标记物,这样的荧光基团一般应具有合适的、高效的(强烈的)激发和发射波长范围。可以修饰荧光基团分子使其能够共价地连接到蛋白、核酸和其他生物分子上。荧光标记的(荧光的)探针广泛地应用于通过荧光显微镜 、流式细胞技术或其他荧光读取设备检测蛋白。 荧光素异硫氰酸酯(FITC)的结构 这种广泛使用的荧光素衍生物染料含有三个环状结构,此结构赋予该分子 荧光特性。对荧光素复合物的修饰是加入了异硫氰酸酯基团,通过该基团可以实现与抗体及其他含有氨
0.05%Tween –2%NGS,振荡,室温静置10min,加20 μl的羊抗小鼠–FITC荧光标记抗血清,稀释度1:100~1:300。孵育于37 ℃ 45 min,加1.25 ml PBS –Tween,室温静置10 min,离心,倾去上清液。 (6)生物素标记探针的荧光显示:加200 μl 4×SSC含0.1%Trion X-100 和5%BSA。室温静置10 min后,加20 μl抗生物素标记FITC抗血清15 μg/ml,孵育在37 ℃ 30 min,以1.5 ml 4×SSc –










