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- 2025年07月13日
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文献和实验Generation of Monoclonal Antibodies to Native Active Human Glycosyltransferases
Complex carbohydrates serve a wide range of biological functions in cells and tissues. Their biosynthesis involves more than 200 distinct glycosyltransferases in human cells, and the expression, properties, and topology of these enzymes
Recombinant Collagen Trimers from Insect Cells and Yeast
have been unsuccessful, but active recombinant human prolyl 4-hydroxylase has been produced in insect cells and yeast by coexpression of its α- and β-subunits (7 ,7 ). We have recently shown that recombinant human type III collagen with a stable triple helix
Expression of Recombinant Antibodies in Mammalian Cell Lines
, and in some cases may require refolding to produce a biologically active product. Another limitation is that recombinant MAbs expressed in bacteria are aglycosylated and can result in the reduction or loss of biological effector functions (3 ). For large
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