BioLegend 643802 Purified anti

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BioLegend 643802 Purified anti-β-actin
产品简介：
产品品牌：BioLegend
产品货号：643802
产品名称：BioLegend 643802 Purified anti-β-actin
产品规格：100 µg

产品说明：
Clone   2F1-1 (See other available formats)
Regulatory Status  RUO
Other Names   Actin, cytoplasmic 1
Isotype   Mouse IgG2b, κ

描述
β-肌动蛋白是一种广泛表达且高度保守的42kD细胞质蛋白，参与细胞运动。这种关键的细胞骨架成分可以被细胞松弛素等药物破坏。由于β-肌动蛋白在所有真核细胞中普遍表达，它经常被用作涉及蛋白质检测的分析（如蛋白质印迹）的负载对照。
β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details
Verified Reactivity   Human, Mouse, Rat
Antibody Type   Monoclonal
Host Species   Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation
The antibody was purified by affinity chromatography.
Concentration   0.5 mg/ml

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application   WB - Quality tested
ICC, IHC-P - Verified

Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes
The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.

Antigen Details
Structure   Actin, cytoplasmic 1
Distribution
Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family    Microfilaments

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