产品说明: Clone 2F1-1 (See other available formats) Regulatory Status RUO Other Names Actin, cytoplasmic 1 Isotype Mouse IgG2b, κ
描述 β-肌动蛋白是一种广泛表达且高度保守的42kD细胞质蛋白,参与细胞运动。这种关键的细胞骨架成分可以被细胞松弛素等药物破坏。由于β-肌动蛋白在所有真核细胞中普遍表达,它经常被用作涉及蛋白质检测的分析(如蛋白质印迹)的负载对照。 β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
Product Details Verified Reactivity Human, Mouse, Rat Antibody Type Monoclonal Host Species Mouse Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography. Concentration 0.5 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Application WB - Quality tested ICC, IHC-P - Verified
Recommended Usage Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.
Antigen Details Structure Actin, cytoplasmic 1 Distribution Ubiquitously expressed in the cytoplasm of all eukaryotic cells
Function Actins are highly conserved proteins that are involved in cell motility Biology Area Cell Biology, Neuroscience, Neuroscience Cell Markers Molecular Family Microfilaments
以下是 BioLegend 品牌部分产品,如需其他产品请联系本公司销售。 BioLegend 405723 Brilliant Violet 510™ anti-mouse IgD 11-26c.2a 50 ug BioLegend 405727 Brilliant Violet 605™ anti-mouse IgD 11-26c.2a 50 ug BioLegend 405729 Alexa Fluor® 700 anti-mouse IgD 11-26c.2a 25 ug BioLegend 405730 Alexa Fluor® 700 anti-mouse IgD 11-26c.2a 100 ug BioLegend 405731 Brilliant Violet 711™ anti-mouse IgD 11-26c.2a 50 ug BioLegend 405733 Biotin anti-mouse IgD 11-26c.2a 50 ug BioLegend 405734 Biotin anti-mouse IgD 11-26c.2a 500 ug BioLegend 405735 PerCP anti-mouse IgD 11-26c.2a 25 ug BioLegend 405736 PerCP anti-mouse IgD 11-26c.2a 100 ug BioLegend 405740 Alexa Fluor® 594 anti-mouse IgD 11-26c.2a 100 ug BioLegend 405744 APC/Fire™ 750 anti-mouse IgD 11-26c.2a 100 ug BioLegend 405749 Spark NIR™ 685 anti-mouse IgD 11-26c.2a 25 ug BioLegend 406205 FITC anti-mouse IgMb AF6-78 50 ug BioLegend 406402 Cyanine3 Donkey anti-rabbit IgG (min. x-reactivity) Poly4064 100 ug BioLegend 406404 DyLight™ 488 Donkey anti-rabbit IgG (min. x-reactivity) Poly4064 100 ug BioLegend 406406 DyLight™ 649 Donkey anti-rabbit IgG (min. x-reactivity) Poly4064 100 ug BioLegend 406414 Alexa Fluor® 647 Donkey anti-rabbit IgG (min. x-reactivity) Poly4064 100 ug BioLegend 406419 Brilliant Violet 510™ Donkey anti-rabbit IgG (min. x-reactivity) Poly4064 50 ug BioLegend 406421 PE Donkey anti-rabbit IgG (min. x-reactivity) Poly4064 100 ug BioLegend 406504 Biotin anti-mouse IgM RMM-1 500 ug BioLegend 406505 FITC anti-mouse IgM RMM-1 50 ug BioLegend 406506 FITC anti-mouse IgM RMM-1 500 ug