BioLegend 640914 FITC Annexin V Apoptosis Detection Kit with PI 产品简介: 产品品牌:BioLegend 产品货号:640914 产品名称:BioLegend 640914 FITC Annexin V Apoptosis Detection Kit with PI 产品规格:100 tests
产品说明: Regulatory Status RUO Other Names Annexin A5 Apoptosis Detection Kit
描述 BioLegend的FITC膜联蛋白V凋亡检测试剂盒具有PI,专门用于识别凋亡和坏死细胞。 膜联蛋白V(或膜联蛋白A5)是细胞内蛋白膜联蛋白家族的一员,以钙依赖的方式与磷脂酰丝氨酸(PS)结合。在健康细胞中,PS通常只在质膜的细胞内小叶上发现,但在早期凋亡过程中,膜不对称性丧失,PS易位到外小叶。荧光染料标记的膜联蛋白V可用于特异性靶向和鉴定凋亡细胞。膜联蛋白V结合缓冲液推荐用于膜联蛋白Ⅴ染色。单独结合膜联蛋白V不能区分凋亡细胞和坏死细胞。为了帮助区分坏死细胞和凋亡细胞,我们建议使用碘化丙啶溶液(PI)。早期凋亡细胞将排除PI,而晚期凋亡细胞和坏死细胞将染色阳性,这是由于这些染料进入细胞核,在细胞核中它们与DNA结合。 碘化丙啶是一种与DNA结合的荧光染料。当被488nm激光激发时,可以在带通滤波器610/10的PE/Texxas Red®通道中进行检测。它通常用于通过流式细胞术评估细胞活力或细胞周期分析中的DNA含量。 BioLegend's FITC Annexin V Apoptosis Detection Kit with PI has been specifically designed for the identification of apoptotic and necrotic cells. Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our Propidium Iodide Solution (PI). Early apoptotic cells will exclude PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA. Propidium iodide is a fluorescent dye that binds to DNA. When excited by 488 nm laser light, it can be detected with in the PE/Texas Red® channel with a bandpass filter 610/10. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.
Product Details Verified Reactivity All mammalian species Concentration Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) Storage & Handling Store between 2°C and 8°C. Do not freeze.
Caution: Propidium Iodide Solution is toxigenic and mutagenic; handle with care. Application FC - Quality tested
Recommended Usage Staining Procedure: 1. Wash cells twice with cold BioLegend's Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/mL. 2. Transfer 100 µL of cell suspension in a 5 mL test tube. 3. Add 5 µL of FITC Annexin V. 4. Add 10 µL of Propidium Iodide Solution. 5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark. 6. Add 400 µL of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.
Antigen Details Biology Area Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
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