BioLegend 582706 Recombinant Human CCL1 (I-309) (carrier-free) 产品简介: 产品品牌:BioLegend 产品货号:582706 产品名称:BioLegend 582706 Recombinant Human CCL1 (I-309) (carrier-free) 产品规格:100 µg
产品说明: Regulatory Status RUO Other Names T lymphocyte-secreted protein I-309
描述 CCL1最初被鉴定为来源于活化的T细胞的分泌蛋白。CCL1/CR8轴参与体内Th2效应细胞向过敏性粘膜炎症部位的募集;结果在过敏性哮喘中CCR8+CD4T细胞数量增加并且在哮喘患者的支气管肺泡灌洗中CCL1浓度高于正常对照。此外,在特应性皮炎患者的血清中检测到高水平的CCL1。CCL1在正常皮肤中组成性表达,并且已经从正常皮肤中分离出CD8和CD4 CCR8+细胞。因此,有人认为CCL1/CR8在皮肤免疫监测中发挥作用。此外,最近的一份出版物显示,CCL1转录物在体外由TGF-β转化为Tregs的CD4中上调,这种转录上调被IL-6逆转。这些数据表明CCL1在Treg转化中起作用。此外,针对CCL1的抗体抑制Tregs的抑制功能。此外,CpG-ODN加抗CCL1在BALB-neuT小鼠中的抗肿瘤作用表明,CCL1的中和可以用作抗肿瘤免疫疗法的佐剂。 CCL1 was initially identified as a secreted protein derived from activated T cells. The CCL1/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation; as a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and a higher concentration of CCL1 in bronchoalveolar lavage of asthmatic patients compared with normal controls. In addition, high levels of CCL1 have been detected in the serum of patients with atopic dermatitis. CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1/CCR8 play a role in skin immunosurveillance. In addition, a more recent publication showed that CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β in vitro, and this transcriptional upregulation is reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Also, antibodies against CCL1 inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.
Product Details Source Human CCL1, amino acids Lys24-Lys96 (Accession# NM_002981.1) was expressed in E. coli.
Molecular Mass The 74 amino acid recombinant protein has a predicted molecular mass of approximately 8.6 kD. The DTT-reduced protein migrates at approximately 13 kD, and the non-reduced protein migrates at approximately 15 kD by SDS-PAGE. The N-terminal amino acid is Lys.
Purity >98%, as determined by Coomassie stained SDS-PAGE.
Formulation 0.22 µm filtered protein solution is in PBS. Concentration 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity Bioactivity was measured by its property to chemoattract mouse BW.5147.G.1.4OUAR.1 cells in a dose dependent manner. The ED50 = 0.5 - 2.5 ng/ml. Application Bioassay
Application Notes BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details Structure Chemokine Distribution T cells, Th1, Th2, diabetogenic T cells, peritoneal macrophages, peritoneal mesothelial cells, Langerhans cells, monocytes, mast cells, NK cells, endothelial cells, and epithelial cells
Function CCL1 chemoattracts CD4 Th2 cells and plays a key role in the progression of Th2 type hypersensitivity
Interaction T cells, Th2 cells, macrophages, microglia, FOXP3 CD4 regulatory T cells, skin-homing CLA+T cells, monocytes, NK cells, dendritic cells, endothelial cells, and smooth muscle cells + Ligand/Receptor CCR8 (CDw198) Biology Area Cell Biology, Signal Transduction Molecular Family Cytokines/Chemokines