产品说明: Clone Poly5343 (See other available formats) Regulatory Status RUO Other Names B cell growth factor 1 (Interleukin 21) Isotype Goat Polyclonal IgG
描述 IL-21通过使用Baf3IL-21R转染子从活化的T细胞的条件培养基中进行功能筛选来鉴定。在正常B细胞中,IL-21可以介导细胞增殖、生长停滞和终末分化或凋亡,这取决于它们的激活状态。IL-21在与活化的CD40抗体孵育后增强B细胞增殖。这一结果表明,IL-21在T和B细胞相互作用后增强了B细胞的功能。然而,IL-21抑制用抗IgM抗体刺激的小鼠和人B细胞的增殖。此外,IL-21增强CD8效应T细胞的增殖、细胞毒性活性和IFN-γ的产生,并诱导活化的自然杀伤(NK)细胞的终末分化。此外,IL-21可以与TGF-β一起驱动Th17反应。然而,IL-21在体外或体内对Th17细胞的分化不是必需的,正如使用IL-21和IL-21R缺陷小鼠所表明的那样。IL-6在TCR刺激下诱导CD4 T细胞产生IL-21。此外,IL-6和IL-21是Tfh分化的关键参与者,其特征是Bcl-6和CXCR5的蛋白表达增加。IL-21R复合物由IL-21R-α链和与其他白细胞介素共享的常见亚基(γc)形成,如IL-2、IL-4、IL-7、IL-9和IL-15。 IL-21 was identified by a functional screening from conditioned medium of activated T cells using Baf3-IL-21R transfectants. In normal B cells, IL-21 can mediate cell proliferation, growth arrest, and terminal differentiation, or apoptosis, depending on their activation status. IL-21 enhances B cell proliferation following incubation with an activating CD40 antibody. This result suggests that IL-21 enhances B cell function following T and B cell interactions. Nevertheless, IL-21 inhibits the proliferation of murine and human B cells stimulated with anti-IgM antibodies. In addition, IL-21 enhances proliferation, cytotoxic activity, and IFN-γ production by CD8-effector T cells, and induces terminal differentiation of activated natural killer (NK) cells. Also, IL-21 can drive Th17 responses in conjunction with TGF-β. Nevertheless, IL-21 is not essential for the differentiation of Th17 cells in vitro or in vivo, as it was showed using IL-21 and IL-21R-deficient mice. IL-6 induces the production of IL-21 from CD4 T cells upon TCR stimulation. In addition, IL-6 and IL-21 are key players in the Tfh differentiation, characterized by increased protein expression of both Bcl-6 and CXCR5. IL-21R complex is formed with the IL-21R-alpha chain and the common subunits (γc) shared with other interleukins, such as IL-2, IL-4, IL-7, IL-9, and IL-15.
Product Details Verified Reactivity Human Antibody Type Polyclonal Host Species Goat Immunogen Recombinant human IL-21 Formulation 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody. Application Neut - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by the neutralization of human IFN-γ production induced by recombinant human IL-21 (5 ng/mL) (Cat. No. 571204) on human NK-92 cells. ND50 range: 0.4 - 2 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application
Antigen Details Structure Interleukin Distribution CD4 activated T cells, Th17 cells, NK T cells Function IL-21 induces T and B cell proliferation, B cell Ig class switching to IgG production, decreases dendritic cell function, enhances differentiation of effector and central memory T cells, and regulates T cell and hematopoietic progenitor cell homeostasis.
Interaction T cells, B cells, NK cells, dendritic cells, and some nonimmune cells, such as fibroblasts and epithelial cells
Ligand/Receptor IL-21R and the common γc subunit (CD132) are components of the IL-21R complex.
Cell Type B cells, Dendritic cells, Hematopoietic stem and progenitors, NKT cells, T cells, Th17, Tregs
Biology Area Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells Molecular Family Cytokines/Chemokines