产品说明: Clone Poly5164 (See other available formats) Regulatory Status RUO Other Names Interleukin-22, IL-10-related T cell-derived inducible factor, IL-TIF Isotype Goat Polyclonal IgG
描述 IL-22是一种在结构上与IL-10相关的细胞因子。小鼠IL-22由179个氨基酸组成,预测分子量为20kD。它主要由Th17、Th1、Th2、淋巴组织诱导细胞(LTi)和自然杀伤细胞亚群分泌。据报道,芳香烃受体(AhR)的表达对TCRγδT细胞产生IL-22至关重要。AhR激活增加Th17极化并诱导IL-22的产生。IL-22通过与异二聚体IL-22受体(IL-22R)复合物结合发挥作用,该复合物由IL-22R1和IL-10Rβ两个受体亚基组成。IL-22作用于非造血组织细胞,如消化和呼吸系统的上皮细胞和皮肤的角质形成细胞。IL-22参与炎症过程,如皮肤炎症、银屑病、炎症性肠病、肝炎和克罗恩病。此外,它在粘膜免疫和伤口愈合过程中发挥着关键作用。 IL-22 is a cytokine structurally related to IL-10. Mouse IL-22 consists of 179 amino acids and has a predicted molecular weight of 20 kD. It is secreted primarily by Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells. It has been reported that aryl hydrocarbon receptor (AhR) expression is essential for the production of IL-22 by TCRγδ T cells. AhR activation increases Th17 polarization and induces IL-22 production. IL-22 functions by engaging the heterodimeric IL-22 receptor (IL-22R) complex, consisting of two receptor subunits, IL-22R1 and IL-10Rβ. IL-22 acts on nonhematopoietic tissue cells, such as epithelial cells of the digestive and respiratory systems and keratinocytes of the skin. IL-22 is involved in inflammatory processes such as dermal inflammation, psoriasis, inflammatory bowel disease, hepatitis, and Crohn’s disease. Moreover, it plays a critical role in mucosal immunity and the wound healing process.
Product Details Verified Reactivity Mouse Antibody Type Polyclonal Host Species Goat Immunogen Mouse IL-22, amino acids Leu34-Val179 (Accession# NM_016971), was expressed in E. coli. Formulation Phosphate-buffered solution, pH 7.2 and 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography. Concentration 0.5 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze. Application ELISA Capture - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.5-2 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-22 recombinant protein ranging from 250-3.9 pg/ml are recommended.
Antigen Details Structure A cytokine structurally related to IL-10 with a predicted molecular weight of 20 kD.
Distribution Th17, Th1, Th2, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells.
Function Acts on nonhematopoietic tissue cells, such as epithelial cells and keratinocytes, involved in inflammatory processes, mucosal immunity, and wound healing. Cell Type Th17, Th1, Th2, NK cells Biology Area Immunology Molecular Family Cytokines/Chemokines