BioLegend 512305 PE anti-human IL-17A 产品简介: 产品品牌:BioLegend 产品货号:512305 产品名称:BioLegend 512305 PE anti-human IL-17A 产品规格:25 tests
产品说明: Clone BL168 (See other available formats) Regulatory Status RUO Other Names Interleukin IL-17A, IL-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8) Isotype Mouse IgG1, κ
描述 IL-17A是IL-17家族的创始成员,IL-17家族由六种结构相关的促炎细胞因子组成。IL-17A 由活化的 CD4+ Th17 细胞亚群分泌,在多种细胞上引发多种生物学活性,包括:诱导上皮、内皮或成纤维细胞中 IL-6、IL-8、G-CSF 和 PGE2 的产生;增强成纤维细胞中ICAM-1的表面表达;NF-κB的激活和T细胞增殖的共刺激。最近的研究表明,在小鼠中,活化的IL-17分泌CD4 +辅助性T细胞(Th17细胞)介导在临床和免疫学上类似于类风湿性关节炎(RA)的自身免疫性关节炎。人IL-17A分别与大鼠IL-17A、小鼠IL-17A和疱疹病毒赛米里(HVS)的ORF13基因编码的蛋白质显示出63%、63%和72%的氨基酸序列同一性。 IL-17A is the founding member of the IL-17 family, a group of six structurally related pro-inflammatory cytokines. IL-17A, secreted by activated CD4+ Th17 cell subpopulation, elicits multiple biological activities on a variety of cells including: the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. Recent studies demonstrated that, in mice, activated IL-17-secreting CD4+ helper T cells (Th17 cells) mediate an autoimmune arthritis that clinically and immunologically resembles rheumatoid arthritis (RA). Human IL-17A shows 63%, 63%, and 72% amino acid sequence identity to rat IL-17A, mouse IL-17A, and a protein encoded by the ORF13 gene of herpesvirus Saimiri (HVS), respectively.
Product Details Isotype Control PE Mouse IgG1, κ Isotype Ctrl (ICFC) Verified Reactivity Human Antibody Type Monoclonal Host Species Mouse Immunogen Recombinant full length human IL-17A Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application ICFC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Antigen Details Structure Cytokine; dimer; ~15 kD (Mammalian) Bioactivity Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells Cell Sources Activated T cell subpopulation Cell Targets Fibroblasts, epithelial and endothelial cells, stromal cells Receptors IL-17R Cell Type Tregs Biology Area Cell Biology, Immunology, Neuroinflammation, Neuroscience Molecular Family Cytokines/Chemokines