IL-1 与两种形式的细胞表面 I 型 (IL-1RI) 和 II 型 (IL-1RII) IL-1 受体结合。IL-1RI 在 T 细胞、成纤维细胞、角质形成细胞、内皮细胞、滑膜衬里细胞、软骨细胞和肝细胞上表达,而 IL-1RII 在 B 细胞谱系、中性粒细胞和骨髓细胞上可见。虽然IL-1α和IL-1β都被IL-1RI和IL-1RII受体识别,但IL-1α与I型受体的结合比IL-1β更好,反之亦然。
BioLegend LEGEND MAX™ 人 IL-1α 夹心酶联免疫吸附测定 (ELISA) 试剂盒包括一个 96 孔试管板,该板预包被小鼠单克隆抗人 IL-1α 捕获抗体。检测抗体为生物素化山羊多克隆抗人IL-1α抗体。该试剂盒专为准确定量来自细胞培养上清液、血清和血浆中的人IL-1α而设计。它通过即用型试剂进行了分析验证。 Interleukin-1 (IL-1) refers to two proteins, IL-1α and IL-1β, which are from different gene products. IL-1α and IL-1β share about 25% amino acid sequence identity. They are initially synthesized as 31 kD precursors, and then cleaved by proteases into mature proteins with molecular mass approximately of 17 kD.
IL-1 binds to both forms of the cell surface type I (IL-1RI) and type II (IL-1RII) IL-1 receptors. IL-1RI is expressed on T cells, fibroblasts, keratinocytes, endothelial cells, synovial lining cells, chondrocytes, and hepatocytes while IL-1RII is found on B-cell lineages, neutrophils, and bone marrow cells. Although both IL-1α and IL-1β are recognized by IL-1RI and IL-1RII receptors, IL-1α binds better to the type I receptor than IL-1β and vice versa.
IL-1 has different biological properties such as immunologic, pro-inflammatory, and protective effects. An increase of IL-1 by a variety of cells is a good indicator of infection by microbial toxins and inflammatory processes. It also affects B cell activation, immunocompetent cells and other immunologic cells. Studies show that the production of IL-1 has been detected in human joint fluids from patients with rheumatoid arthritis, osteoarthritis, traumatic arthritis, and psoriatic arthritis. In addition, IL-1α shows elevated levels in cancer such as colon cancer and cervical cancer.
The BioLegend LEGEND MAX™ Human IL-1α Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) kit includes a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human IL-1α capture antibody. The detection antibody is a biotinylated goat polyclonal anti-human IL-1α antibody. This kit is specifically designed for the accurate quantitation of human IL-1α from cell culture supernatant, serum, and plasma. It is analytically validated with ready-to-use reagents.
Product Details Verified Reactivity Human Application ELISA
Antigen Details Cell Sources Macrophage Biology Area Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience Molecular Family Cytokines/Chemokines