产品说明: Clone AD2 (See other available formats) Regulatory Status RUO Workshop V B-CD73.3 Other Names Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT Isotype Mouse IgG1, κ
描述 CD73 是一种 70 kD 糖磷脂酰肌醇 (GPI) 连接的 5'-核苷酸酶,也称为 ecto-5'-核苷酸酶。它将单磷酸腺苷(AMP)转化为腺苷。CD73 在 T 细胞和 B 细胞亚群、间充质干细胞、滤泡树突状细胞、内皮细胞和上皮细胞上表达。据报道,CD73可刺激T细胞活化,并介导淋巴细胞与滤泡树突状细胞和内皮细胞的粘附。 CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
Product Details Isotype Control PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl Verified Reactivity Human Reported Reactivity African Green, Baboon Antibody Type Monoclonal Host Species Mouse Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm) Application Notes Additional reported applications (for the relevant formats) include:immunofluorescence3.
Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.
Additional Product Notes BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Antigen Details Structure GPI-linked 5'-nucleotidase, 70 kD Distribution Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
Function Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells Cell Type B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs Biology Area Costimulatory Molecules, Immunology, Stem Cells Molecular Family Adhesion Molecules, CD Molecules