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99
- 英文名：
PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)
- 供应商：
北京云肽生物科技有限公司
BioLegend 344009 PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)
北京云肽生物科技有限公司作为 BioLegend 的特约经销商，为广大客户提供原装正品，货期短，折扣好的服务准则。
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BioLegend 344009 PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)
产品简介：
产品品牌：BioLegend
产品货号：344009
产品名称：BioLegend 344009 PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)
产品规格：25 tests
产品说明：
Clone AD2 (See other available formats)
Regulatory Status RUO
Workshop V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype Mouse IgG1, κ
描述
CD73 是一种 70 kD 糖磷脂酰肌醇 （GPI） 连接的 5'-核苷酸酶，也称为 ecto-5'-核苷酸酶。它将单磷酸腺苷（AMP）转化为腺苷。CD73 在 T 细胞和 B 细胞亚群、间充质干细胞、滤泡树突状细胞、内皮细胞和上皮细胞上表达。据报道，CD73可刺激T细胞活化，并介导淋巴细胞与滤泡树突状细胞和内皮细胞的粘附。
CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.
Product Details
Isotype Control PE/Cyanine7 Mouse IgG1, κ Isotype Ctrl
Verified Reactivity Human
Reported Reactivity African Green, Baboon
Antibody Type Monoclonal
Host Species Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
Additional reported applications (for the relevant formats) include:immunofluorescence3.
Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.
Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Antigen Details
Structure GPI-linked 5'-nucleotidase, 70 kD
Distribution
Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells
Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area Costimulatory Molecules, Immunology, Stem Cells
Molecular Family Adhesion Molecules, CD Molecules
以下是 BioLegend 品牌部分产品，如需其他产品请联系本公司销售。
BioLegend 302612 PE/Cyanine7 anti-human CD25 BC96 100 tests
BioLegend 302613 APC/Cyanine7 anti-human CD25 BC96 25 tests
BioLegend 302614 APC/Cyanine7 anti-human CD25 BC96 100 tests
BioLegend 302618 Alexa Fluor® 647 anti-human CD25 BC96 100 tests
BioLegend 302624 Biotin anti-human CD25 BC96 100 ug
BioLegend 302625 PerCP/Cyanine5.5 anti-human CD25 BC96 25 tests
BioLegend 302626 PerCP/Cyanine5.5 anti-human CD25 BC96 100 tests
BioLegend 302629 Brilliant Violet 421™ anti-human CD25 BC96 25 tests
BioLegend 302630 Brilliant Violet 421™ anti-human CD25 BC96 100 tests
BioLegend 302631 Brilliant Violet 605™ anti-human CD25 BC96 25 tests
BioLegend 302633 Brilliant Violet 650™ anti-human CD25 BC96 25 tests
BioLegend 302634 Brilliant Violet 650™ anti-human CD25 BC96 100 tests
BioLegend 302636 Brilliant Violet 711™ anti-human CD25 BC96 100 tests
BioLegend 302642 APC/Fire™ 750 anti-human CD25 BC96 100 tests
BioLegend 302646 PE/Dazzle™ 594 anti-human CD25 BC96 100 tests
BioLegend 302702 Purified anti-human CD26 BA5b 100 ug
BioLegend 302704 FITC anti-human CD26 BA5b 100 tests
BioLegend 302705 PE anti-human CD26 BA5b 25 tests
BioLegend 302709 APC anti-human CD26 BA5b 25 tests
BioLegend 302710 APC anti-human CD26 BA5b 100 tests
BioLegend 302714 PE/Cyanine7 anti-human CD26 BA5b 100 tests
BioLegend 302715 PerCP/Cyanine5.5 anti-human CD26 BA5b 25 tests
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文献和实验相关实验
Cell Surface Immunofluorescence Staining Protocol
实验试剂 Cell Staining Buffer [ Details:BioLegend Cat. #420201] Red Cell Lysis Buffer [ Details: BioLegend Cat. #420301] 7-AAD Viability Staining Solution[ Details: BioLegend Cat. #420403] TruStain FcX™[ Details: anti
) A431 cells. At 48 h after infection, these cells were stained with ten different primary antibodies (5 g/ml) specific for different cell surface markers: anti-CD15 (clone 2F3; BD, PharMingen, San Diego, CA), anti-ERBB-2 (clone SP77; ref. 5), anti-ERBB
Methods for the Detection of D-Amino-Acid Oxidase
. Proced. Online 1998;1:27-31. doi:10.1251/bpo7 Submitted: February 26, 1998; Published: May 14, 1998. Indexing terms: d amino acid oxidase; methods
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BioLegend 344009 PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase)
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