产品说明: Clone 5E10 (See other available formats) Regulatory Status RUO Workshop HCDM listed Other Names Thy-1, Thy1 Isotype Mouse IgG1, κ
描述 CD90是一种25-35kD的GPI锚定蛋白,也称为Thy-1。它属于Ig超家族。人CD90在神经元细胞、CD34+细胞的亚群、胎儿肝细胞和胎儿胸腺细胞的亚集、成纤维细胞、活化的内皮细胞和一些白血病细胞系上表达。CD34+CD90+细胞是原始的造血干细胞。据报道,Thy-1与β2和β3整合素结合,在调节细胞粘附和突起生长中发挥双峰作用,并抑制造血干细胞的增殖和分化 CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34+ cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34+CD90+ cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.
Product Details Isotype Control PerCP/Cyanine5.5 Mouse IgG1, κ Isotype Ctrl Verified Reactivity Human Reported Reactivity African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig Antibody Type Monoclonal Host Species Mouse Immunogen HEL cells Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. Application Notes Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of *-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.
Additional Product Notes BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Antigen Details Structure 25-35 kD glycoprotein, Ig superfamily Distribution Subset of CD34+ hematopoietic stem cells, subset of fetal thymocytes, subset of fetal liver cells, fibroblast, activated endothelial cells, neurons and some leukemia cell lines
Function Regulate hematopoiesis and neural cell growth, cell adhesion Ligand/Receptor β3 integrin, β2 integrin Cell Type Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors, Leukemia, Mesenchymal Stem Cells, Neurons, Thymocytes Biology Area Immunology, Stem Cells Molecular Family CD Molecules