产品说明: Clone WM59 (See other available formats) Regulatory Status RUO Workshop V P025 Other Names PECAM-1, EndoCAM Isotype Mouse IgG1, κ
描述 CD31 是一种 130-140 kD 的 I 型跨膜糖蛋白,也称为血小板内皮细胞粘附分子-1 (PECAM-1) 或 Endocam。它在单核细胞、血小板、粒细胞、内皮细胞和淋巴细胞亚群中表达。据报道,CD31 可结合 CD38 并在炎症情况下参与伤口愈合、血管生成和细胞迁移。 CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.
Product Details Isotype Control Brilliant Violet 605™ Mouse IgG1, κ Isotype Ctrl Verified Reactivity Human, Cynomolgus, Rhesus Reported Reactivity African Green, Baboon Antibody Type Monoclonal Host Species Mouse Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). Preparation The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions. Concentration Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.) Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. Excitation Laser Violet Laser (405 nm) Application Notes Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of *-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).
The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.
Antigen Details Structure Ig superfamily, type I transmembrane glycoprotein, 130-140 kD Distribution Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset
Function Cell adhesion, signal transduction Ligand/Receptor CD38 Cell Type Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets Biology Area Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience Molecular Family Adhesion Molecules, CD Molecules
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