产品说明: Clone GL7 (See other available formats) Regulatory Status RUO Other Names Ly77, T and B cell activation marker Isotype Rat IgM, κ
描述 GL7抗原,也称为Ly77,是一种35kD的蛋白质。GL7抗原具有一个表位,该表位包含GL7抗体识别的非硫酸化α2-6-唾液酸-LacNAc。GL7抗原由前B细胞和未成熟B细胞、活化的T细胞和B细胞以及约20%的TCR明亮胸腺细胞表达。激活后在小鼠脾细胞上上调。它可能在调节或粘附中发挥作用。GL7高表达的B细胞显示出更高的抗体产生和抗原呈递能力。 The GL7 antigen, also known as Ly77, is a 35 kD protein. The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes. It is upregulated on mouse splenocytes following activation. It may play a role in regulation or adhesion. GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.
Product Details Verified Reactivity Mouse, Human Antibody Type Monoclonal Host Species Rat Immunogen LPS activated DBA/J mouse B cells Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. Concentration 0.2 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.
Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
Additional Product Notes BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions
Antigen Details Structure 35 kD Distribution Germinal center B cells, activated B and T cells
Function Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase Ligand/Receptor Neu5Ac-recognizing lectins Cell Type B cells, T cells Biology Area Immunology, Innate Immunity Molecular Family CD Molecules
以下是BioLegend 品牌部分产品,如需其他产品请联系本公司销售。 BioLegend 125410 Alexa Fluor® 488 anti-mouse/human Mac-2 (Galectin-3) M3/38 100 ug BioLegend 125420 APC anti-mouse/human Mac-2 (Galectin-3) M3/38 100 ug BioLegend 125502 Purified anti-mouse H-2 M1/42 100 ug BioLegend 125506 PE anti-mouse H-2 M1/42 100 ug BioLegend 125607 Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1) MC-480 25 tests BioLegend 125618 APC anti-mouse/human CD15 (SSEA-1) MC-480 100 tests BioLegend 126105 FITC anti-mouse CD22 OX-97 50 ug BioLegend 126109 APC anti-mouse CD22 OX-97 25 ug BioLegend 126111 PE anti-mouse CD22 OX-97 25 ug BioLegend 126112 PE anti-mouse CD22 OX-97 100 ug BioLegend 126309 PE anti-mouse CD357 (GITR) DTA-1 25 ug BioLegend 126310 PE anti-mouse CD357 (GITR) DTA-1 100 ug BioLegend 126311 APC anti-mouse CD357 (GITR) DTA-1 25 ug BioLegend 126317 PE/Cyanine7 anti-mouse CD357 (GITR) DTA-1 25 ug BioLegend 126322 Ultra-LEAF™ Purified anti-mouse CD357 (GITR) DTA-1 1 mg BioLegend 126401 Purified anti-mouse FOXP3 MF-14 25 ug BioLegend 126402 Purified anti-mouse FOXP3 MF-14 100 ug BioLegend 126403 PE anti-mouse FOXP3 MF-14 25 ug BioLegend 126404 PE anti-mouse FOXP3 MF-14 100 ug BioLegend 126405 Alexa Fluor® 488 anti-mouse FOXP3 MF-14 25 ug BioLegend 126406 Alexa Fluor® 488 anti-mouse FOXP3 MF-14 100 ug BioLegend 126407 Alexa Fluor® 647 anti-mouse FOXP3 MF-14 25 ug