产品说明: Clone E18 (See other available formats) Regulatory Status RUO Other Names Tp44, T44 Isotype Mouse IgG2b, κ 描述 CD28是一种44kD糖蛋白,也称为Tp44或T44。它是Ig超家族的一员,在胸腺细胞、大多数外周T细胞和NK细胞上表达。与CD80(B7-1)和CD86(B7-2)相关,CD28作为T和NK细胞活化和增殖的第二信号。E18抗体阻断37.51抗体与CD28的结合,并共刺激T细胞活化和增殖。 CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The E18 antibody blocks the binding of 37.51 antibody to CD28 and costimulates T cell activation and proliferation.
Product Details Isotype Control FITC Mouse IgG2b, κ Isotype Ctrl Verified Reactivity Mouse Antibody Type Monoclonal Host Species Mouse Immunogen A20 cells and recombinant mCD28Ig Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. Concentration 0.5 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Excitation Laser Blue Laser (488 nm) Application Notes Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 122004).
Antigen Details Structure Ig superfamily, 44 kD Distribution Thymocytes, CD4+, CD8+ peripheral T cells, NK cells Function Costimulates T and NK cells Ligand/Receptor CD80 (B7-1), CD86 (B7-2) Cell Type NK cells, T cells, Thymocytes, Tregs Biology Area Costimulatory Molecules, Immunology Molecular Family CD Molecules
以下是BioLegend 品牌部分产品,如需其他产品请联系本公司销售。 BioLegend 105045 APC/Fire™ 750 anti-mouse CD86 GL-1 25 ug BioLegend 105046 APC/Fire™ 750 anti-mouse CD86 GL-1 100 ug BioLegend 105052 Ultra-LEAF™ Purified anti-mouse CD86 GL-1 1 mg BioLegend 105106 PE anti-mouse CD86 PO3 200 ug BioLegend 105109 FITC anti-mouse CD86 PO3 50 ug BioLegend 105110 FITC anti-mouse CD86 PO3 500 ug BioLegend 105114 APC anti-mouse CD86 PO3 100 ug BioLegend 105115 PE/Cyanine7 anti-mouse CD86 PO3 25 ug BioLegend 105122 Alexa Fluor® 700 anti-mouse CD86 PO3 100 ug BioLegend 105123 Brilliant Violet 421™ anti-mouse CD86 PO3 50 ug BioLegend 105304 Biotin anti-mouse CD90.2 (Thy1.2) 30-H12 500 ug BioLegend 105305 FITC anti-mouse CD90.2 (Thy1.2) 30-H12 50 ug BioLegend 105306 FITC anti-mouse CD90.2 (Thy1.2) 30-H12 500 ug BioLegend 105307 PE anti-mouse CD90.2 (Thy1.2) 30-H12 50 ug BioLegend 105308 PE anti-mouse CD90.2 (Thy1.2) 30-H12 200 ug BioLegend 105311 APC anti-mouse CD90.2 (Thy1.2) 30-H12 25 ug BioLegend 105312 APC anti-mouse CD90.2 (Thy1.2) 30-H12 100 ug BioLegend 105316 Alexa Fluor® 488 anti-mouse CD90.2 (Thy1.2) 30-H12 100 ug BioLegend 105321 PerCP anti-mouse CD90.2 (Thy1.2) 30-H12 25 ug BioLegend 105322 PerCP anti-mouse CD90.2 (Thy1.2) 30-H12 100 ug BioLegend 105326 PE/Cyanine7 anti-mouse CD90.2 (Thy1.2) 30-H12 100 ug BioLegend 105327 APC/Cyanine7 anti-mouse CD90.2 (Thy1.2) 30-H12 25 ug