BioLegend 119307 PE anti-mouse CD34 产品简介: 产品品牌:BioLegend 产品货号:119307 产品名称:BioLegend 119307 PE anti-mouse CD34 产品规格:50 µg
产品说明: Clone MEC14.7 (See other available formats) Regulatory Status RUO Other Names Mucosialin Isotype Rat IgG2a, κ 描述 CD34是一种高度糖基化的造血祖细胞抗原。据报道,CD34的两种亚型是通过选择性剪接产生的。这种抗原在造血祖细胞、内皮细胞、大脑和睾丸上表达。CD34被认为是早期造血祖细胞的粘附分子,介导干细胞与细胞外基质或基质细胞的粘附。CD34在丝氨酸残基上被PKC磷酸化。 CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.
Product Details Isotype Control PE Rat IgG2a, κ Isotype Ctrl Verified Reactivity Mouse Antibody Type Monoclonal Host Species Rat Immunogen Cells transfected with mouse CD34 Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. Concentration 0.2 mg/ml Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm) Application Notes The MEC14.7 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting6, and immunohistochemistry of *-fixed frozen sections and paraffin-embedded sections2,4,5,6.
Antigen Details Structure Type I membrane protein, 75-120 kD, highly glycosylated; two isoforms reported Distribution Hematopoietic progenitors, brain, testis, endothelial cells; low expression in thymus, spleen, and bone marrow Function Possible adhesion molecule thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Presents carbohydrate ligands to selectins. Ligand/Receptor L-selectin, other selectins Cell Type Endothelial cells, Hematopoietic stem and progenitors Biology Area Cell Biology, Immunology, Neuroinflammation, Neuroscience Molecular Family Adhesion Molecules, CD Molecules
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