This assay employs the competitive enzyme immunoassay technique. Determine the level of Dog E2(Estradiol) in the sample. Add standard and sample to the microplate that have been pre-coated with Dog E2(Estradiol) antigen. After incubation, add Biotin-conjugated anti-Dog E2(Estradiol) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Dog E2(Estradiol) in the sample was proportional to the OD value. The concentration of Dog E2(Estradiol) in the sample can be calculated by drawing a standard curve.
灵敏度
1.88 pg/mL
Sensitivity
1.88 pg/mL
检测范围
3.12-200 pg/mL
Examination range
3.12-200 pg/mL
特异性
可检测样本中的狗雌二醇,且与其类似物无明显交叉反应。
Specificity
This kit recognizes Dog E2(Estradiol) in samples. No significant cross-reactivity or interference between Human ApoB(Apolipoprotein B) and analogues was observed.Dog E2(Estradiol) in samples. No significant cross-reactivity or interference between Dog E2(Estradiol) and analogues was observed.
Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%.
回收率
分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。
Recovery
A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate.