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大鼠
大鼠
大鼠
用于大鼠血清、血浆或其他相关生物液体中基质金属蛋白酶13的测定
双抗体夹心法酶联免疫吸附检测技术
0.31-20 ng/mL
96 TESTS
FLIGHT
48TESTS/96TESTS
规格: | 48TESTS | 产品价格: | ¥1900.0 |
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规格: | 96TESTS | 产品价格: | ¥2800.0 |
工作原理 |
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Purpose | This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Rat MMP-13(Matrix Metalloproteinase 13) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Rat MMP-13(Matrix Metalloproteinase 13) antibody. After incubation, add Biotin-conjugated anti-Rat MMP-13(Matrix Metalloproteinase 13) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Rat MMP-13(Matrix Metalloproteinase 13) in the sample was proportional to the OD value. The concentration of Rat MMP-13(Matrix Metalloproteinase 13) in the sample can be calculated by drawing a standard curve.antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Mouse ADP/Acrp30(Adiponectin) in the sample was proportional to the OD value. The concentration of Mouse ADP/Acrp30(Adiponectin) in the sample can be calculated by drawing a standard curve. | |
灵敏度 | 0.19 ng/mL | |
Sensitivity | 0.19 ng/mL | |
检测范围 | 0.31-20 ng/mL | |
Examination range | 0.31-20 ng/mL | |
特异性 | 可检测样本中的大鼠基质金属蛋白酶13,且与其类似物无明显交叉反应。 | |
Specificity | This kit recognizes Rat MMP-13(Matrix Metalloproteinase 13) in samples. No significant cross-reactivity or interference between Rat MMP-13(Matrix Metalloproteinase 13) and analogues was observed. | |
重复性 |
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Repeatability | Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%. | |
回收率 | 分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。 | |
Recovery | A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate. |
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样本收集及储存等资料.docx 附 (下载 0 次)