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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 英文名:
GeneChip DNA Labeling Reagent, 7.5 mM
- 供应商:
北京云肽生物科技有限公司
我们北京云肽生物科技有限公司作为Applied Biosystems公司的特约经销商为客户提供,正规进口,保证原装正品,货期稳定,折扣好的服务标准。
Applied Biosystems公司为生命科学和实验室仪器领域全球最大的生产厂家,2004年公司全球销售额达17亿美圆,公司为美国纽约证交所上市公司。 Applied Biosystems公司致力于发展生命科学技术的创新,Applied Biosystems公司与广大生命科学家一起不断努力,为人类创造更好的医疗健康及生存环境。
Applied Biosystems900542 GeneChip DNA Labeling Reagent, 7.5 mM,产品简介:
产品品牌:Applied Biosystems
产品货号:900542
产品名称:GeneChip DNA Labeling Reagent, 7.5 mM
产品规格:1 kit
Applied Biosystems900542 GeneChip DNA Labeling Reagent, 7.5 mM,产品说明:
The GeneChip DNA Labeling Reagent is used with the Prokaryotic Target Labeling Assay for 3' expression studies with GeneChip prokaryotic Brand Arrays. The assay utilizes reverse transcriptase and random hexamer primers to produce DNA complementary to the RNA. The cDNA products are then fragmented by DNase I and labeled with terminal transferase and biotinylated GeneChip DNA Labeling Reagent at the 3' termini.
Functionally tested on GeneChip Brand Arrays, we recommend the Prokaryotic Target Labeling Assay and associated reagents for use in prokaryotic gene expression studies.
Prokaryotic Target Labeling Assay components include:
• Control Oligo B2, 3nM
• GeneChip DNA Labeling Reagent
• GeneChip Poly-A RNA Control Kit
Detailed instructions for using this reagent are described in Section 3, Chapter 1 of the GeneChip Expression Analysis Technical Manual.
Each kit contains 1 vial of 60 µL of the GeneChip DNA Labeling Reagent, 7.5 mM, sufficient for 30 labeling reactions.
产品订购信息:
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Applied Biosystems 900542 GeneChip DNA Labeling Reagent, 7.5 mM 1 kit
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0.5 ml 100% ethanol 4. DNA Wash 1 ml 75% ethanol (2X) 5. DNA Solubilization 8 mM NaOH ethanol and 8 mM NaOH
Site‐Specific Protein Labeling with SNAP‐Tags
and specificities and provide flexibility not available with fluorescent proteins. The SNAP?tag is a modified form of the DNA repair enzyme human O 6 ?alkylguanine?DNA?alkyltransferase, and undergoes a self?labeling reaction to form a covalent bond with O
Southern Blotting:DNA Transfer
Immunochemiluminescence: Blot Wash #1: 2x SSC; 0.1% (w/v) SDS. Blot Wash #2: 0.1x SSC; 0.1% (w/v) SDS. Buffer #1: 100mM maleic acid, pH 7.5 150mM NaCl Buffer #2: 2% Blocking Reagent in Buffer
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