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- 百生跃(Bioleaper)
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- BR2010386
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
1年
- 英文名：
Anti-Integrin b7 / ITGB7 Reference Antibody (Genentech patent anti-Integrin β7)
- 库存：
999
- 供应商：
百生跃(Bioleaper)
- 规格：
50μg
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文献和实验Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
Figure 19.14.3 Immobilization of anti‐β2µ‐globulin antibody to the chip surface. Each step and corresponding response is labeled at the top of the sensorgram. Each X on the response represents a report point
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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