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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商:
齐源生物
- 库存:
999
- 靶点:
CD4
- 级别:
抗人 CD4流式抗体荧光标记PE
- 目录编号:
抗人 CD4流式抗体荧光标记PE
- 克隆性:
单克隆
- 抗原来源:
抗人 CD4流式抗体荧光标记PE
- 保质期:
二年
- 抗体英文名:
anti-human CD4 Antibody PE
- 抗体名:
CD4
- 标记物:
PE
- 宿主:
小鼠
- 适应物种:
人
- 免疫原:
抗人 CD4流式抗体荧光标记PE
- 亚型:
抗人 CD4流式抗体荧光标记PE
- 形态:
溶液
- 应用范围:
FC
- 保存条件:
2-8℃
- 浓度:
0.2 mg/mL
- 规格:
25 tests/100 tests/500 tests/100 µg
| 规格: | 25 tests | 产品价格: | ¥500.0 |
|---|---|---|---|
| 规格: | 100 tests | 产品价格: | ¥1200.0 |
| 规格: | 500 tests | 产品价格: | ¥5900.0 |
| 规格: | 100 µg | 产品价格: | ¥2400.0 |
Clone:RPA-T4 (See other available formats)
Regulatory Status:RUO
Workshop:IV T114
Other Names:T4
Isotype:Mouse IgG1, κ
Ave. Rating:Submit a Review
Product Citations:39 publications
CD4,也称为 T4,是一种 55 kD 单链 I 型跨膜糖蛋白,在大多数胸腺细胞、T 细胞亚群和单核细胞/巨噬细胞上表达。CD4 是 Ig 超家族的成员,识别与 MHC II 类分子相关的抗原,并参与细胞间相互作用、胸腺分化和信号转导。CD4 是 HIV 的主要受体,与 HIV gp120 结合。CD4 还被证明可以与 IL-16 相互作用。
产品详细:
Verified ReactivityHumanReported ReactivityChimpanzeeAntibody TypeMonoclonalHost SpeciesMouseFormulationµg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).PreparationThe antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.Concentrationµg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)Storage & HandlingThe antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation LaserBlue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of a、cetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
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- 作者
- 内容
- 询问日期
文献和实验1、Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
2、Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
3、Deng MC, et al. 1995. Circulation 91:1647. (IHC)
4、Friedm/an T, et al. 1999. J. Immunol. 162:5256. (IHC)
5、Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
6、Lan RY, et al. 2006. Hepatology 43:729.
7、Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
8、Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
9、Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
10、Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
11、Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
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抗体二抗上连接有荧光染料。直接标记染色背景低,实验程序少尽量减少实验工序和过程,以保证实验的真实和准确性。因此在条件允许的范围内,建议尽量用直接标记的抗体进行实验。 3、流式抗体荧光标记的选择: 如果实验中检测单一指标:不同荧光标记在不同的仪器上强度不同。以某仪器为例:PE >APC >PE-Cy5 >PerCP >FITC >PerCP-Cy5.5,通常来说,PE最强,适用于弱表达抗原。FITC强度较弱,适用于强表达抗原,使用范围比较广。用户需根据检测的目标蛋白进行具体选择。 如果同时检测多个
#01 什么是同型对照? 同型对照(Isotype Control),是指与使用的流式抗体具有相同种属来源、亚型、荧光标记物、使用剂量和浓度,但对目标靶点无特异性结合的抗体。同型对照通常用来消除抗体与抗原的非特异性结合造成的背景染色。 #02 为什么要做同型对照? 流式抗体与靶标的结合,是通过抗体的 Fab 端与靶标 Marker 进行特异性结合而完成的。白细胞(除T细胞外)表面大多表达 CD16、CD32、CD64 等 Fc 受体,这些受体可与各种抗体的 Fc 端非特异性结合,出现非特异
接收范围。 一般来说,实验panel有几种荧光,就需要设置几个单阳管。 同型对照(Isotype Control),是指与使用的流式抗体具有相同种属来源、亚型、荧光标记物、使用剂量和浓度,但对目标靶点无特异性结合的抗体。同型对照通常用来消除抗体的非特异性结合造成的背景染色。 FMO 对照是 Fluorescence Minus One(荧光扣除一)的缩写。在多色实验中,可能会出现某些通道信号难以区分的情况,主要原因是其他某个荧光对该通道的干扰很大,不能使用未染色的空白对照或全部用同型对照来设门











