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ATTO 700 Biotin
MCE 国际站：ATTO 700 Biotin
产品活性：ATTO 700 Biotin 是 ATTO 700 的生物素衍生物，最大激发/发射波长：700/716 nm。
研究领域：Others
作用靶点：Fluorescent Dye
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文献和实验Immunohistochemisty-Fluorescence Protocol
antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted 1:500) in Wash Buffer-Saponin. Wash the cells twice in Wash Buffer-Saponin. Incubate the centrifuged cell pellet
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
Expression L19 using Pichia pastoris
nitrogen base) 4x10-5 biotin 1% methanol Dissolve 10g yeast extract and 20g peptone in 700ml water. Autoclave 20 min on liquid cycle. Cool to room temperature then add the following and mix well: 100 ml 1M potassium phophate buffer pH 6 100
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