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- 2025年07月15日
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Please store the product under the recommended conditions in the Certificate of Analysis.
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货期：电询
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MedChemExpress LLC
- CAS号：
129273-26-3
- 规格：
电询
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Digoxigenin NHS ester
CAS No. : 129273-26-3
MCE 国际站：Digoxigenin NHS ester
产品活性：Digoxigenin NHS ester 是一种活化酯，在温和条件下很容易与氨基(NH2)发生反应，将 digoxigenin (HY-B1025) 部分连接到蛋白质或氨基上。Digoxigenin NHS ester 可用于标记蛋白质和寡核苷酸。
研究领域：Others
作用靶点：Others
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文献和实验Coupling Peptides To Resin For Affinity Purification
5% ethylene diamine in water. Incubate 15' at RT. Wash with 10 volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate
Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
Labeling Tubulin and Quantifying Labeling Stoichiometry
that are not clear, using DMSO instead of glycerol for the first polymerization step appears to increase the labeling stoichiometry by ~25% for C2CF-SNHS ester. For labeling with rhodamine (tetramethylrhodamine NHS ester) and X-rhodamine, we generally use glycerol
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