AHR agonist 4,2953023-30-6

Cell-Based Assays for Identification of Aryl Hydrocarbon Receptor (AhR) Activators

and potency of the inducing chemical. The AhR agonist activity of positive test chemicals can be confirmed by demonstrating their ability to stimulate expression of CYP1A1, an endogenous AhR-responsive gene, using quantitative real-time PCR. The detailed

DNA Binding (Gel Retardation Assay) Analysis for Identification of Aryl Hydrocarbon (Ah) Receptor Agonists and Antagonists

. GRA of the aryl hydrocarbon receptor (AhR) relies on the in vitro ability of the cytosolic AhR protein complex to convert into its high affinity DNA binding form following its interaction with and activation by an AhR agonist and, as such, the GRA

Flow Cytometry Assay to Measure Internalization of GPCRs

onto 6-cm dishes.2) Fill dishes with 2 mL growth media with 30 mM HEPES, pH 7.4. Set aside a dish of 293/B2AR at 4degC. You will use this dish to estimate the total number of receptors on the surface of a sample (i.e. before agonist treatment). HEPES reduces