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- 2025年12月05日
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2408994-22-7
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BET-IN-15
CAS No. : 2408994-22-7
MCE 国际站：BET-IN-15
产品活性：BET-IN-15 (compound 1) 是一种有效的且具有口服活性的 BET 抑制剂，对于 BRD4-BD1, BRD4-BD2 的 IC50 值分别为 0.64、0.25 nM。BET-IN-15 显示出抗增殖活性。
研究领域：Epigenetics
作用靶点：Epigenetic Reader Domain
In Vitro: BET-IN-15 (compound 1) (72 h) shows antiproliferative activity with IC50s of 3.14, 1.56 nM for MV4-11, Kasumi-1 cellS, respectively.
In Vivo: BET-IN-15 (compound 1) (72 h) 显示出抗增殖活性，对于 MV4-11、Kasumi-1 细胞的IC50s 分别为 3.14、1.56 nM。
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文献和实验Phosphoamino acid analysis:Mark Kamps's method
of PAA standards.15 min should be plenty. 13.Mark the plate with radioactive ink so that you can extrapolate where the origins were and can align the film unambigously with the plate and the PAA markers. 14.Expose the plate to flashed film with a screen
Chromatin Immunoprecipitation (ChIP)
, for aspiration; see Step 20) Ice Incubators preset to 55°C, 65°C Microcentrifuge Micropipettor with tips PCR apparatus Rotator Sonicator Spectrophotometer Spin column (QIAGEN) Tubes (conical, 15-mL) Tubes
Phosphoamino acid analysis:Mark Kamps method
. If you are using another rig, you will have to optimize your electrophoresis times. 11. Dry plate in oven. 12. Spray plate with ninhydrin sol'n for several seconds. Incubate in oven until can see purple spots of PAA standards. 15 min should be plenty
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