- ￥3500
- Tanda
- 78.13-5000 pg/mL
- 中国
- TD711324
- 2025年11月27日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
800
- 供应商：
武汉天德
- 检测范围：
78.13-5000 pg/mL
- 检测方法：
ELISA
- 应用：
用于人血清、血浆或其他相关生物液体中磷酸化神经丝H的测定。
- 适应物种：
Human
- 标记物：
FITC
- 样本：
Human
- 规格：
96Test
TD711324
人磷酸化神经丝H(pNF-H)ELISA试剂盒
| 英文名 | Human pNF-H ELISA Kit | ||
| 别名 | Neurofilament Heavy Polypeptide;Neurofilament Triplet H Protein;200 KDa Neurofilament Protein;NF-H;NFH;KIAA0845;CMT2CC | ||
| 相关类别 | ELISA | 储存 | 冷藏（2-8℃） |
应用
用于人血清、血浆或其他相关生物液体中磷酸化神经丝H的测定。
For the determination of Human pNF-H (Phosphorylated Neurofilament H) concentrations in serum, plasma and other related biological fluids.
属性
| 工作原理 | 本试剂盒采用的是双抗体夹心法酶联免疫吸附检测技术（ELISA）。测定样品中人磷酸化神经丝H水平。向预先包被了抗人磷酸化神经丝H抗体的酶标孔中，加入标准品和样本，温育后，加入生物素标记的抗磷酸化神经丝H抗体。再与HRP标记的链霉亲和素结合，形成免疫复合物，再经过温育和洗涤，去除未结合的酶，然后加入显色底物TMB，产生蓝色，并在酸的作用下转化成最终的黄色。最后，在450 nm处测定反应孔样品吸光度（OD）值，样本中的人磷酸化神经丝H浓度与OD值成正比，通过绘制标准曲线计算出样本中人磷酸化神经丝H的浓度。 |
| Purpose | This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Human pNF-H (Phosphorylated Neurofilament H) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Human pNF-H (Phosphorylated Neurofilament H) antibody. After incubation, add Biotin-conjugated anti-Human pNF-H (Phosphorylated Neurofilament H) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Human pNF-H (Phosphorylated Neurofilament H) in the sample was proportional to the OD value. The concentration of Human pNF-H (Phosphorylated Neurofilament H) in the sample can be calculated by drawing a standard curve. |
| 灵敏度 | 46.88 pg/mL |
| Sensitivity | 46.88 pg/mL |
| 检测范围 | 78.13-5000 pg/mL |
| Examination range | 78.13-5000 pg/mL |
| 特异性 | 可检测样本中的人磷酸化神经丝H，且与其类似物无明显交叉反应。 |
| Specificity | This kit recognizes Human pNF-H (Phosphorylated Neurofilament H) in samples. No significant cross-reactivity or interference between Human pNF-H (Phosphorylated Neurofilament H) and analogues was ob/served. |
| 重复性 | 三种不同浓度的样本在同一块板子上分别检测20次，以及在不同的板子上分别检测20次，板内和板间变异系数均小于10%。 |
| Repeatability | Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%. |
| 回收率 | 分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。 |
| Recovery | A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate. |
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Anti-Factor H Autoantibodies Assay
of autoantibodies directed against Factor H (FH), the main plasmatic alternative complement pathway regulatory protein. Their presence induces an acquired functional FH deficiency. The anti-FH autoantibodies screening must be performed at the very onset
抗体的结构 抗体是能与抗原特异性结合的免疫球蛋白(immunoglobulin,Ig)。Ig 分五类，即 IgG、IgA、IgM、IgD 和 IgE。与免疫测定有关的 Ig 主要为 IgG 和 IgM。Ig 由两个轻链（L）和两个重链（H）的单体组成。Ig 的轻链是相同的，有 κ（kappa）和 λ（Lambda）两种型别。五类 Ig 的重链结构不同，这决定了它们的抗原性也不同。IgG 和 IgM 的重链分别称为 γ（gamma）链和 μ（mu ）链。 重链和轻链的 N 端的氨基酸排列顺序
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