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- 详细信息
- 文献和实验
- 技术资料
- 库存:
800
- 供应商:
武汉天德
- 检测范围:
0.16-10 ng/mL
- 检测方法:
ELISA
- 应用:
用于人血清、血浆或其他相关生物液体中细胞程序性死亡蛋白1配体1的测定。
- 适应物种:
Human
- 标记物:
FITC
- 样本:
Human
- 规格:
96Test
TD711209
人细胞程序性死亡蛋白1配体1(PDCD1LG1)ELISA试剂盒
| 英文名 | Human PDCD1LG1 ELISA Kit | ||
| 别名 | CD274; B7-H1; PD-L1; PDCD1L1; PDL1; B7 homolog 1 | ||
| 相关类别 | ELISA | 储存 | 冷藏(2-8℃) |
应用
用于人血清、血浆或其他相关生物液体中细胞程序性死亡蛋白1配体1的测定。
For the determination of Human PDCD1LG1(Programmed Cell Death Protein 1 Ligand 1) concentrations in serum, plasma and other related biological fluids.
属性
| 工作原理 | 本试剂盒采用的是双抗体夹心法酶联免疫吸附检测技术(ELISA)。测定样品中人细胞程序性死亡蛋白1配体1水平。向预先包被了抗人细胞程序性死亡蛋白1配体1抗体的酶标孔中,加入标准品和样本,温育后,加入生物素标记的抗细胞程序性死亡蛋白1配体1抗体。再与HRP标记的链霉亲和素结合,形成免疫复合物,再经过温育和洗涤,去除未结合的酶,然后加入显色底物TMB,产生蓝色,并在酸的作用下转化成最终的黄色。最后,在450 nm处测定反应孔样品吸光度(OD)值,样本中的人细胞程序性死亡蛋白1配体1浓度与OD值成正比,通过绘制标准曲线计算出样本中人细胞程序性死亡蛋白1配体1的浓度。 |
| Purpose | This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Human PDCD1LG1(Programmed Cell Death Protein 1 Ligand 1) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Human PDCD1LG1(Programmed Cell Death Protein 1 Ligand 1) antibody. After incubation, add Biotin-conjugated anti-Human PDCD1LG1(Programmed Cell Death Protein 1 Ligand 1) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Human PDCD1LG1(Programmed Cell Death Protein 1 Ligand 1) in the sample was proportional to the OD value. The concentration of Human PDCD1LG1(Programmed Cell Death Protein 1 Ligand 1) in the sample can be calculated by drawing a standard curve. |
| 灵敏度 | 0.1 ng/mL |
| Sensitivity | 0.1 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| Examination range | 0.16-10 ng/mL |
| 特异性 | 可检测样本中的人细胞程序性死亡蛋白1配体1,且与其类似物无明显交叉反应。 |
| Specificity | This kit recognizes Human PDCD1LG1(Programmed Cell Death Protein 1 Ligand 1) in samples. No significant cross-reactivity or interference between Human PDCD1LG1(Programmed Cell Death Protein 1 Ligand 1) and analogues was ob/served. |
| 重复性 | 三种不同浓度的样本在同一块板子上分别检测20次,以及在不同的板子上分别检测20次,板内和板间变异系数均小于10%。 |
| Repeatability | Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%. |
| 回收率 | 分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。 |
| Recovery | A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate. |
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文献和实验文献速递:个性化循环肿瘤 DNA 可成为免疫治疗疗效新型biomarker
研究背景 使用程序性死亡-1抑制剂或其配体(抗PD-1/PD-L1)的免疫检查点阻断 (ICB) 疗法已成为治疗许多癌症的有效方法。然而仅有少数患者对ICB疗法表现出长期良好的反应,大多数患者对免疫检查点阻断剂都没有阳性反应或因为阻断剂的不良反应而不得不停止服用。虽然已经发现了许多类别的生物标志物,但目前仍未发现强有力的治疗反应预测标志物。寻求新的ICB反应的非侵入性生物标志物能大大提高ICB疗法的临床效果。 研究内容及结果 2020年9月,在Nature子刊Nature Cancer
因子 >>> 分装 ELISA 试剂盒 货号 品名 方法 规格 价格 备注 F00010 血管紧张素转化酶 (Human ACE) ELISA 48T/96T 2000/3000 进口分装 F00020 激活素 A(Activin A) ELISA 48T/96T 2000/3000 进口分装 F00021 雄激素结合蛋白 (ABP) ELISA 48T/96T 1800/2800 进口分装 F00022 促肾上腺皮质激素 (ACTH) ELISA 48T/96
人细胞因子 >>> 分装ELISA 试剂盒 货号 品名 方法 规格 价格 备注 F0001 人血管紧张素转化酶(Human ACE) ELISA 48T/96T 2000/3000 进口分装 F0002 激活素A(Activin A) ELISA 48T/96T 2000/3000 进口分装 F0003 ★ 人脂联素(human Adiponectin) ELISA 48T/96
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