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- 详细信息
- 文献和实验
- 技术资料
- 库存:
800
- 供应商:
武汉天德
- 检测范围:
3.13-200 pg/mL
- 检测方法:
ELISA
- 应用:
用于人血清、血浆或其他相关生物液体中全段甲状旁腺素的测定
- 适应物种:
Human
- 标记物:
FITC
- 样本:
Human
- 规格:
96Test
TD711201
人全段甲状旁腺素(I-PTH)ELISA试剂盒
| 英文名 | Human I-PTH ELISA Kit | ||
| 别名 | iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin | ||
| 相关类别 | ELISA | 储存 | 冷藏(2-8℃) |
应用
用于人血清、血浆或其他相关生物液体中全段甲状旁腺素的测定
For the determination of Human I-PTH(intact Parathormone) concentrations in serum, plasma and other related biological fluids.
属性
| 工作原理 | 本试剂盒采用的是双抗体夹心法酶联免疫吸附检测技术(ELISA)。测定样品中人全段甲状旁腺素水平。向预先包被了抗人全段甲状旁腺素抗体的酶标孔中,加入标准品和样本,温育后,加入生物素标记的抗全段甲状旁腺素抗体。再与HRP标记的链霉亲和素结合,形成免疫复合物,再经过温育和洗涤,去除未结合的酶,然后加入显色底物TMB,产生蓝色,并在酸的作用下转化成最终的黄色。最后,在450 nm处测定反应孔样品吸光度(OD)值,样本中的人全段甲状旁腺素浓度与OD值成正比,通过绘制标准曲线计算出样本中人全段甲状旁腺素的浓度。 |
| Purpose | This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Human I-PTH(intact Parathormone) in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Human I-PTH(intact Parathormone) antibody. After incubation, add Biotin-conjugated anti-Human I-PTH(intact Parathormone) antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Human I-PTH(intact Parathormone) in the sample was proportional to the OD value. The concentration of Human I-PTH(intact Parathormone) in the sample can be calculated by drawing a standard curve. |
| 灵敏度 | 1.88 pg/mL |
| Sensitivity | 1.88 pg/mL |
| 检测范围 | 3.13-200 pg/mL |
| Examination range | 3.13-200 pg/mL |
| 特异性 | 可检测样本中的人全段甲状旁腺素,且与其类似物无明显交叉反应。 |
| Specificity | This kit recognizes Human I-PTH(intact Parathormone) in samples. No significant cross-reactivity or interference between Human I-PTH(intact Parathormone) and analogues was ob/served. |
| 重复性 | 三种不同浓度的样本在同一块板子上分别检测20次,以及在不同的板子上分别检测20次,板内和板间变异系数均小于10%。 |
| Repeatability | Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%. |
| 回收率 | 分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白,做回收实验,得出回收率范围和平均回收率。 |
| Recovery | A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate. |
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文献和实验北京时间 2022 年 2 月 9 日,中国科学院深圳先进技术研究院脑认知与脑疾病研究所、深港脑科学创新研究院杨帆课题组在 Nature Communications 上发表题为 An optogenetic approach for regulating human parathyroid hormone secretion 的最新研究成果。 研究团队历时 5 年,创新性地将光遗传技术运用于甲状旁腺素(Parathyroid Hormone, PTH)的分泌调控,通过精准节律性调节
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人甲状旁腺激素 (I-PTH)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 I-PTH 单抗包被于酶标板上,标准品和样品中的 I-PTH与单抗结合,加入生物素化的抗人 I-PTH
甲状旁腺素(PTH)是甲状旁腺主细胞合成和分泌的多肽激素。它通过骨和肾来实现升高血钙,降低血磷的作用:①对骨的作用。PTH能动员骨钙入血,使血钙升高。它可作用于骨细胞膜系统,促使Ca 2 转运入细胞外液,这是PTH动员骨钙入血的快速效应,仅数分钟内即可发生。此外,还可加强破骨细胞的活动,使骨钙大量入血,这一效应为延缓效应,在PTH作用后12~14小时开始,几天或几周后达高峰。两个效应互相配合,不仅能迅速升高血钙,而且能维持很长时间。②对肾的作用。PTH能抑制近球小管对磷酸
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