- ￥3500
- Tanda
- 1.56-100 ng/mL
- 中国
- TD711403
- 2025年12月05日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
800
- 供应商：
武汉天德
- 检测范围：
1.56-100 ng/mL
- 检测方法：
ELISA
- 应用：
用于人血清、血浆或其他相关生物液体中原癌基因RET的测定
- 适应物种：
Human
- 标记物：
FITC
- 样本：
血清、血浆及相关液体
- 规格：
96Test
TD711403
人原癌基因RET(RET)ELISA试剂盒
| 英文名 | Human RET(Ret Proto-Oncogene) ELISA Kit | ||
| 相关类别 | ELISA | 储存 | 按标签提示分开储存 |
应用
用于人血清、血浆或其他相关生物液体中原癌基因RET的测定
For the determination of Human RET concentrations in serum, plasma and other related biological fluids.
属性
| 工作原理 | 本试剂盒采用的是双抗体夹心法酶联免疫吸附检测技术（ELISA）。测定样品中人原癌基因RET水平。向预先包被了抗人原癌基因RET抗体的酶标孔中，加入标准品和样本，温育后，加入生物素标记的抗原癌基因RET抗体。再与HRP标记的链霉亲和素结合，形成免疫复合物，再经过温育和洗涤，去除未结合的酶，然后加入显色底物TMB，产生蓝色，并在酸的作用下转化成最终的黄色。最后，在450 nm处测定反应孔样品吸光度（OD）值，样本中的人原癌基因RET浓度与OD值成正比，通过绘制标准曲线计算出样本中人原癌基因RET的浓度。 |
| Purpose | This assay employs the Sandwich enzyme immunoassay technique. Determine the level of Human RET in the sample. Add standard and sample to the microplate that have been pre-coated with Anti-Human RET antibody. After incubation, add Biotin-conjugated anti-Human RET antibody. It is then combined with HRP-conjugated streptavidin to form an immune complex, then incubated and washed to remove unbound enzyme, and then added to the chromogenic substrate TMB to produce a blue color, and converted to the final yellow under the action of acid. Finally, the absorbance (OD) value was measured at 450 nm. The concentration of Human RET in the sample was proportional to the OD value. The concentration of Human RET in the sample can be calculated by drawing a standard curve. |
| 灵敏度 | 0.94 ng/mL |
| Sensitivity | 0.94 ng/mL |
| 检测范围 | 1.56-100 ng/mL |
| Examination range | 1.56-100 ng/mL |
| 特异性 | 可检测样本中的人原癌基因RET，且与其类似物无明显交叉反应。 |
| Specificity | This kit recognizes Human RET in samples. No significant cross-reactivity or interference between Human ApoB(Apolipoprotein B) and analogues was obs/erved.Human RET in samples. No significant cross-reactivity or interference between Human RET and analogues was obs/erved. |
| 重复性 | 三种不同浓度的样本在同一块板子上分别检测20次，以及在不同的板子上分别检测20次，板内和板间变异系数均小于10%。 |
| Repeatability | Three different concentrations of samples were detected 20 times on the same board and 20 times on different boards. Within the plate or between the plates, the coefficient of variation is <10%. |
| 回收率 | 分别往稀释不同倍数的5个样本中加入已知浓度的目标蛋白，做回收实验，得出回收率范围和平均回收率。 |
| Recovery | A known concentration of the target protein was added to 5 samples which were diluted to different multiples, and a recovery experiment was performed to obtain a recovery range and an average recovery rate. |
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文献和实验kelizi 哪位大侠知道RET原癌基因的结构域，其相应的定位、功能？ 以及相应蛋白在细胞的定位及功能？如何调节？ 谢谢！如果有相应的文献资料更佳 woxingwosu http://www.genecards.org/cgi-bin/carddisp.pl?gene=RET&search=ret 应该比较全了，你在从里面的链接可以找到更多~~ kelizi
challenge. Rearrangements of the RET oncogene have been proposed as a marker for papillary thyroid cancer. In this chapter, methods for the analysis of the RET oncogene in laser microdissected papillary thyroid cancer tissue are described.
脂肪燃烧的指令从哪里来？Nature 揭示神经免疫互作如何与
脂肪和 MSCs 中均观察到了 Gdnf 的表达降低。此外，在纯化的 MSCs 中刺激 ADRB2 可导致 MSCs 来源的 Gdnf 增加，PDGFRA 阳性的内脏脂肪细胞与 GDNF 在空间上也存在共定位。图片来源：Nature前期的研究表明，GDNF 家族配体及其受体 GFRα 能够激活神经系统、肾脏和造血细胞亚群中的酪氨酸激酶受体 RET。研究人员也在内脏脂肪 ILC2s 中观察到较高水平的 RET。为了研究这种受体酪氨酸激酶在脂肪 ILC2s 中的作用，他们特异删除了造血细胞中的 Ret，结果发现
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