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- 文献和实验
- 技术资料
- 供应商:
联科生物
- 库存:
99
- 样本:
血清,血浆,细胞培养上清及其他生物学样本
- 标记物:
HRP
- 适应物种:
Human/Mouse/Rat
- 应用:
ELISA
- 检测方法:
竞争法
- 检测范围:
15.63 pg/mL - 2000 pg/mL
- 规格:
96T/48T
| 规格: | 96T | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 48T | 产品价格: | ¥2500.0 |
描述
| 商品名 |
Prostaglandin E2/PGE2 Competitive ELISA Kit (前列腺素E2 ELISA试剂盒) |
|---|---|
| 检测方法 |
竞争法 |
| 精密度 |
板内变异系数:3.9% - 4.3%;板间变异系数:5.2% - 6.5% |
| 样本类型 |
血清,血浆,细胞培养上清及其他生物学样本 |
| 检测样本体积 |
100 μL(稀释液) |
| 灵敏度 |
1.08 pg/mL |
| 检测范围 |
15.63 pg/mL - 2000 pg/mL |
| 回收率 |
92 - 102% |
| 平均回收率 |
0.99 |
| 板式 |
96孔板,可拆 |
| 保存 |
试剂盒未拆开,-20℃保存。已拆开,标准品、抗体、偶联物和酶-20℃保存,其它4℃保存。 |
| 运输条件 |
干冰运输 |
| 组分 |
|
检测原理:本试剂盒采用固相竞争酶联免疫吸附检测技术。兔抗小鼠抗体预包被在高亲和力的酶标板上。PGE2特异性的单克隆抗体加入至孔中,经过孵育,与固相抗体结合。洗涤后,加入生物素标记的PGE2和未标记的PGE2或样本,竞争结合单克隆抗体上有限的结合位点。洗涤去除未结合的物质后,加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后,加入显色底物,颜色反应的深浅与PGE2的浓度成反比。加入终止液终止反应,在450 nm波长(参考波长570 - 630 nm)测定吸光度值。本试剂盒已验证人、小鼠和大鼠血清样本,但其它哺乳动物的PGE2预计也可被检测。
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上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人前列腺素E2 ( PGE2 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液等生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 PGE2 单抗包被于酶标板上,标准品和样品中的 PGE2与单抗结合,加入生物素化的抗人 PGE2
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 兔前列腺素E2 ( PGE2 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗兔 PGE2 单抗包被于酶标板上,标准品和样品中的 PGE2与单抗结合,加入生物素化的抗兔 PGE2 ,形成
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 鸡前列腺素 E2(PGE2)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液等生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗鸡 PGE2 单抗包被于酶标板上,标准品和样品中的 PGE2 与单抗结合,加入生物素化的抗鸡 PGE2 ,形成免疫复合物连接在板上,辣根
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