产品简介:
BioDynami NGS单链DNA建库试剂盒(illumina platform)是一种以剪切单链DNA (50 ng - 500 ng)为输入构建高质量文库的工具。该试剂盒是制作含有变性DNA、病毒DNA、高度降解的古DNA和其他单链DNA样本的NGS文库的理想工具。ssDNA试剂盒的工作流程很简单:分两步制作文库,接下去是PCR和清理步骤。这些文库可在1.5小时内准备好,只需10分钟的实践时间。库取决于索引的类型。
The NGS Single Stranded DNA Library Prep Kit (illumina platform) was developed for construction of high quality libraries using sheared single stranded DNA (50 ng – 500 ng) as input. The kit is ideal for making NGS libraries with samples of denatured DNA, viral DNA, highly degraded ancient DNA and other single stranded DNA. The workflow of the ssDNA kit is simple: make the libraries in two steps followed by PCR and cleanup steps. The libraries will be ready in just 1.5 hours with a 10 minutes of hands-on time. The library pooling is possible dependent on the type of index.
BioDynami NGS单链DNA建库试剂盒工作流程
试剂盒有三种index类型:
Non-index(Cat.# 30081):没有索引。
Index(Cat.# 30082):每个索引引物都有一个独特的6个碱基的索引序列,可以用来识别库。ssDNA文库复用多达48个样本。
Unique dual index(Cat.# 30083):ssDNA样本复用多达96个库是可能的唯一的双索引。BioDynami建立了一个Four-Base Difference Index System。这使得可以生成在8个碱基索引长度中至少有4个碱基不同的索引。唯一的双索引引物消除了NGS错误(例如:解复用错误、读取错误赋值、索引跳变等)。
Three index types are available for the kit:
Non-index (Cat.# 30081): Libraries do not have index.
Index(Cat.# 30082): Each of the index primers has a unique 6-base index sequence that can be used to identify libraries. ssDNA library multiplexing is up to 48 samples.
Unique dual index (Cat.# 30083): ssDNA sample multiplexing up to 96 libraries is possible with unique dual indexes. We have developed a Four-Base Difference Index System. This makes it possible to generate indexes with at least 4 bases different from each other in the 8 bases index length. The unique dual indexing primers remove NGS errors (example: de-multiplexing errors, read mis-assignment, index hopping etc).
试剂盒优势:
★ 快速简单的实验方案
★ 简单的程序
★ 减少磁珠:减少50%以上用于清理步骤的磁珠
★ 单链DNA作为输入:从50 ng到500 ng
★ 快速:总操作时间仅为1.5小时
★ 简单:动手时间只有10分钟
★ 准备使用的主混合物:简化反应设置
★ 反应组分少:简化反应准备
Kit advantages:
-
-
- Quick and simple Protocol
- Quick: Total protocol time is only 1.5 hours
- Simple: Hands-on time only 10 minutes
- Easy procedure based on:
- The ready-to-use master mix: Made reaction setup easy
- Less reaction components: Simplify the reaction preparation
- Less magnetic beads needed: Reduced more than 50% of the beads for cleanup steps
- Single stranded DNA as input: From 50 ng to 500 ng
BioDynami NGS单链DNA建库试剂盒与常规文库准备试剂盒相比,具有相似的文库转换效率和产量
比对率和重复率:单链DNA建库试剂盒与双链DNA建库试剂盒的比较
产品订购:
产品名称 |
货号 |
产品规格 |
NGS Single Stranded DNA Library Prep Kit |
30081S |
non-index,24 reactions |
NGS Single Stranded DNA Library Prep Kit |
30081L |
non-index,48 reactions |
NGS Single Stranded DNA Library Prep Kit |
30082S |
index,24 reactions |
NGS Single Stranded DNA Library Prep Kit |
30082L |
index,48 reactions |
NGS Single Stranded DNA Library Prep Kit |
30083S |
unique dual index,96 reactions |
NGS Single Stranded DNA Library Prep Kit |
30083L |
unique dual index,192 reactions |