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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Pig Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Pig Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1), partial品名简称:
Recombinant Pig UCHL1 protein, partial货号:
CSB-EP747573PI规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
UCHL1Uniprot No.:
Q6SEG5别名:
(UCH-L1)(Ubiquitin thioesterase L1)种属:
Sus scrofa (Pig)蛋白长度:
Partial来源:
E.coli分子量:
28.7 kDa表达区域:
1-220aa氨基酸序列:
MQLKPMEINPEMLNKVLTRLGVAGHWRFADVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLEFEDGSVLKQFLSETEKLSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDSLLQDAAKVCREFTEREQGEVRFSAVALC蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.功能2:
/文献:
"Cloning and expression of ubiquitin carboxyl-terminal hydrolase in pig." Tian X., Li J., Chen Y. Submitted (NOV-2003)研究领域:
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文献和实验(Ubiquitin C-terminal hydrolase-L1,UCH-L1)是一种主要分布于脑内,同时对人类大脑具有高度特异性的球蛋白。 癫痫大发作组脑脊液 UCH-L1 水平显著高于部分性发作组和对照组。癫痫患者的脑脊液 UCHL1 水平与癫痫发作的严重程度和癫痫发作的持续时间密切相关。癫痫发作后 6h 内检验患者血清 UCH-L1 及 S100β 水平,结果显示癫痫组 UCHL-1 及 S100β 水平显著高于精神源性非癫痫性发作组及健康人群组,精神源性非癫痫性发作组 S100β 水平显著高于健康对照
spermatogonia coexpress GPR125, integrin, alpha 6 (ITGA6), THY1 (also known as CD90), GFRA1, and ubiquitin carboxyl-terminal esterase L1 (UCHL1), markers for rodent or pig SSCs/progenitors, suggesting that GPR125- and GFRA1-positive spermatogonia
A Method for Assaying Deubiquitinating Enzymes
-soluble radioactivity increases in an incubation time-dependent fashion and this increase can be completely blocked by the treatment of Ub-aldehyde. Since YUH1 as well as other DUBs are known to specifically cleave the carboxyl side of the C-terminal Gly
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