ANGPT2蛋白|斯克罗法ANGPT2蛋白|斯克罗法ANGP

Research Areas

Cardiovascular

Uniprot ID

Q9BDY7

Gene Names

ANGPT2

Alternative Name(s)

ANG-2

Lead Time

13-23 business days

Organism

Sus scrofa (Pig)

Source

E.coli

Expression Region

19-496aa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Target Protein Sequence

YNNFRKSMDSTGKRQYQVQHGPCSYTFLLPETDNCRSPSSSYVSNAVQRDAPLDYDDSVRRLQVLENIMENNTQWLMKLESYIQDNMKKEMVEIQQNAVQNQTAVMIEIGTNLLNQTAEQTRKLTDVEAQVLNQTTRLELQLLEHSLSTNKLEKQILDQTSEINKLQDKNSFLEKKVLDMEDKHIVQLQSIKEEKDQLQVLVSKQNSIIEELEKQLVTATVNNSVLQKQQHDLMETVHNLLTMISTSNSAKHSLVAKEEQIIFRDCAEAFKSGLTTSGTYTLTFPNSTEETKAYCDMETGGGGWTVIQRREDGSVDFQRTWKEYKMGFGSPSGEHWLGNEFVSQVTNQKRYVLKIHLRDWEGNEAYSLYEHFYLSSEEFNYRIHLKGLTGTAGKISSISQPGNDFSTKDADNDKCICKCSQMLTGGWWFDACGPSNLNGMYYPQRQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF

MW

60.9 kDa

Purity

Greater than 85% as determined by SDS-PAGE.

Endotoxin

Not test.

Biological_Activity

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Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Involved in the regulation of lymphangiogenesis.

Function

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