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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Vaccinia virus Late transcription elongation factor H5 (VACWR103)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Vaccinia virus Late transcription elongation factor H5 (VACWR103)品名简称:
Recombinant Vaccinia virus VACWR103 protein货号:
CSB-MP4550VAI规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
VACWR103Uniprot No.:
P07242别名:
(Viral late gene transcription factor 4 )(VLTF-4)种属:
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))蛋白长度:
Full Length来源:
Mammalian cell分子量:
51.2 kDa表达区域:
1-203aa氨基酸序列:
MAWSITNKADTSSFTKMAEIRAHLKNSAENKDKNEDIFPEDVIIPSTKPKTKRATTPRKPAATKRSTKKEEVEEEVVIEEYHQTTEKNSPSPGVSDIVESVAAVELDDSDGDDEPMVQVEAGKVNHSARSDLSDLKVATDNIVKDLKKIITRISAVSTVLEDVQAAGISRQFTSMTKAITTLSDLVTEGKSKVVRKKVKTCKK蛋白标签:
C-terminal hFc1-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
18-28 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Involved in the co-transcriptional or post-transcriptional endoribonucleolytic cleavage that generates sequence-homogeneous 3' ends during late transcription. Involved in postreplicative transcription elongation on intermediate and late genes (Probable). Also involved in DNA replication and in multiple steps of virion morphogenesis. Required both for inclusion of virosoplasm into crescents as well as for maturation of immature virions (IV) into mature virions (MV).功能2:
/文献:
"Purification and properties of the vaccinia virus mRNA processing factor." D'Costa S.M., Bainbridge T.W., Condit R.C. J. Biol. Chem. 283:5267-5275(2008)研究领域:
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文献和实验An In Vitro Transcription System for Studying Vaccinia Virus Late Genes
This chapter describes a protocol that allows accurate in vitro transcription of vaccinia virus late genes. In this method, extracts are made from vaccinia virus-infected cells and used as enzyme sources to produce mRNAs from plasmid templates
An In Vitro Transcription System for Studying Vaccinia Virus Early Genes
Transcription of the vaccinia virus early genes occurs within the confines of the virion core structure. Therefore, isolated virions are a particularly rich source of proteins that function in early mRNA biosynthesis. Methods are described
A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus
genome to accommodate large genes (>20 kb pairs), availability of insertional sites on the viral genome yielding viable recombinant viruses, ability of the vaccinia virus to infect a wide range of mammalian hosts, cytoplasmic transcription and replication
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