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- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Serine protease 1 (PRSS1)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Serine protease 1 (PRSS1)品名简称:
Recombinant Human PRSS1 protein货号:
CSB-YP018811HU规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
PRSS1Uniprot No.:
P07477别名:
(Anionic trypsin I)(Beta-trypsin)(Cationic trypsinogen)(Pretrypsinogen I)(Trypsin I)(TRP1)(TRY1)(TRYP1)种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
25.6 kDa表达区域:
24-247aa氨基酸序列:
IVGGYNCEENSVPYQVSLNSGYHFCGGSLINEQWVVSAGHCYKSRIQVRLGEHNIEVLEGNEQFINAAKIIRHPQYDRKTLNNDIMLIKLSSRAVINARVSTISLPTAPPATGTKCLISGWGNTASSGADYPDELQCLDAPVLSQAKCEASYPGKITSNMFCVGFLEGGKDSCQGDSGGPVVCNGQLQGVVSWGDGCAQKNKPGVYTKVYNYVKWIKNTIAANS蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
25-35 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates.功能2:
/文献:
"Gene conversion between functional trypsinogen genes PRSS1 and PRSS2 associated with chronic pancreatitis in a six-year-old girl." Teich N., Nemoda Z., Koehler H., Heinritz W., Moessner J., Keim V., Sahin-Toth M. Hum. Mutat. 25:343-347(2005)研究领域:
Metabolism请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验问:大家好,我要做牛胰蛋白酶的表达,但是还没有找到相关的文献,有谁知道他的序列,是223个氨基酸残基。现在这谢谢大家了!答:看看是不是这个!LOCUS NM_001113727 879 bp mRNA linear MAM 22-JAN-2008DEFINITION Bos taurus cationic trypsin (LOC780933), mRNA.ACCESSION NM_001113727 XM_584594VERSION NM_001113727.1 GI:165973957
Growing feeder-independent embryonic stem cells§
) without calcium and magnesium (Gibco-BRL). 2 mercaptoethanol stock solution: Add 70 µl of beta-mercaptoethanol (Sigma) to 20 ml of distilled, deionized water (Gibco-BRL). Filter sterilize and store at 4°C for up to 2 weeks. ES cell medium: 1× GMEM
In Vitro Reconstitution of CFTR Biogenesis and Degradation
consist of � subunits, three of which exhibit distinct protease activities. These Pup1/Z/beta2 , Pre2/X/beta5 , and Pre3/Y/beta1 subunits preferentially cleave substrates after basic, hydrophobic, or acidic residues, respectively (4 ,5 ). These cleavage
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