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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bacillus subtilis DNA polymerase I (polA), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Bacillus subtilis DNA polymerase I (polA), partial品名简称:
Recombinant Bacillus subtilis polA protein, partial货号:
CSB-EP520296BRJ2规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
polAUniprot No.:
O34996别名:
(POL I)种属:
Bacillus subtilis (strain 168)蛋白长度:
Partial来源:
E.coli分子量:
72.8 kDa表达区域:
297-880aa氨基酸序列:
DQSLEDINVKTVTDVTSDILVSPSAFVVEQIGDNYHEEPILGFSIVNETGAYFIPKDIAVESEVFKEWVENDEQKKWVFDSKRAVVALRWQGIELKGAEFDTLLAAYIINPGNSYDDVASVAKDYGLHIVSSDESVYGKGAKRAVPSEDVLSEHLGRKALAIQSLREKLVQELENNDQLELFEELEMPLALILGEMESTGVKVDVDRLKRMGEELGAKLKEYEEKIHEIAGEPFNINSPKQLGVILFEKIGLPVVKKTKTGYSTSADVLEKLADKHDIVDYILQYRQIGKLQSTYIEGLLKVTRPDSHKVHTRFNQALTQTGRLSSTDPNLQNIPIRLEEGRKIRQAFVPSEKDWLIFAADYSQIELRVLAHISKDENLIEAFTNDMDIHTKTAMDVFHVAKDEVTSAMRRQAKAVNFGIVYGISDYGLSQNLGITRKEAGAFIDRYLESFQGVKAYMEDSVQEAKQKGYVTTLMHRRRYIPELTSRNFNIRSFAERTAMNTPIQGSAADIIKKAMIDMAAKLKEKQLKARLLLQVHDELIFEAPKEEIEILEKLVPEVMEHALALDVPLKVDFASGPSWYDAK蛋白标签:
C-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Component of a mechanosensitive channel required for rapidly adapting mechanically activated (MA) currents. Required for Merkel-cell mechanotransduction. Plays a major role in light-touch mechanosensation.功能2:
/文献:
"Loss of stretch-activated channels, PIEZOs, accelerates non-small cell lung cancer progression and cell migration." Huang Z., Sun Z., Zhang X., Niu K., Wang Y., Zheng J., Li H., Liu Y. Biosci Rep 39:BSR20181679-BSR20181679(2019)研究领域:
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文献和实验又称枯草芽孢杆菌。一种分布普遍的腐生细菌,容易从土壤微生物中分离得到。细胞杆状,大小为0.7~ 0.8× 2~ 3微米。革兰氏阳性反应。细胞周身具多条鞭毛。能产生芽孢,椭圆形到柱状,位于菌体中央或一端。细胞分裂后产生的细胞可以连成丝状。菌落粗糙,不透明,无闪光,扩张,污白色或微黄色。枯草杆菌可用做制取淀粉酶和蛋白酶,如枯草杆菌 Bf7658是生产淀粉酶的主要菌种;枯草杆菌 AS1.398是生产中性蛋白酶的主要菌种。淀粉酶在纺织、食品和发酵工业上应用广泛,蛋白酶在鞣革、轻工和医药工业上有
出版社,2001,P403,P407,P409 风疹病毒(Rubella virus,RUV)Genome为单链RNA,其Genome3’第6389位核苷酸至亚基因组RNA(subgenomic RNA,24S RNA)起始位点。Sindis virus的基因组的相应区域参与亚基因组RNA的合成。RUV基因组的24 S RNA由3327个核苷酸组成(不包括PolA尾),其5’末端和3’末端同样具有帽状结构和PolA尾,而且被甲基化。24 S RNA与病毒基因组3’相对应,编码病毒的3个结构蛋白,其排列顺序
在细胞中存在的数目不多,是促进DNA链延长的主要酶。 发现 在50年代的中期,A. Kornberg和他的同事们就想到DNA的复制必然是一种酶的催化作用,于是决心分离出这种酶并研究其结构和作用机制。为了达到这个目的,他们分离的蛋白,然后加到体外合成系统中即同位素标记的dNTP、Mg2+及模板DNA,经过大量的工作,于1956年终于发现了DNA聚合酶Ⅰ(DNA polymerase Ⅰ,DNA pol Ⅰ)原来称为Kornberg酶。以后又相续发现了DNA pol Ⅱ和DNA
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