AMFR蛋白|人AMFR蛋白|人AMFR重组蛋白|Homo

Research Areas

Cancer

Uniprot ID

Q9UKV5

Gene Names

AMFR

Alternative Name(s)

(Autocrine motility factor receptor)(AMF receptor)(RING finger protein 45)(gp78)

Lead Time

3-7 business days

Organism

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

515-601aa

Protein Length

Partial

Tag Info

C-terminal hFc-tagged

Target Protein Sequence

NSPVERPSSDQEEGETSAQTERVPLDLSPRLEETLDFGEVEVEPSEVEDFEARGSRFSKSADERQRMLVQRKDELLQQARKRFLNKS

MW

39.0 kDa

Purity

Greater than 85% as determined by SDS-PAGE.

Endotoxin

Not test.

Biological_Activity

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Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Storage

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Relevance

E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation . Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD) . The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane . In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination . The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction . In addition to ubiquitination on lysine residues, catalyzes ubiquitination on cysteine residues: together with INSIG1, mediates polyubiquitination of SOAT2/ACAT2 at 'Cys-277', leading to its degradation when the lipid levels are low . Catalyzes ubiquitination and subsequent degradation of INSIG1 when cells are depleted of sterols . Mediates polyubiquitination of INSIG2 at 'Cys-215' in some tissues, leading to its degradation . Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex . Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation . Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor . In association with LMBR1L and UBAC2, negatively regulates the canonical Wnt signaling pathway in the lymphocytes by promoting the ubiquitin-mediated degradation of CTNNB1 and Wnt receptors FZD6 and LRP6 .

Function

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