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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Rat Glial fibrillary acidic protein (Gfap)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Rat Glial fibrillary acidic protein (Gfap)品名简称:
Recombinant Rat Gfap protein货号:
CSB-EP009369RAa0规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
GfapUniprot No.:
P47819别名:
(GFAP)种属:
Rattus norvegicus (Rat)蛋白长度:
Full Length来源:
E.coli分子量:
55.9 kDa表达区域:
1-430aa氨基酸序列:
MERRRITSARRSYASSETMVRGHGPTRHLGTIPRLSLSRMTPPLPARVDFSLAGALNAGFKETRASERAEMMELNDRFASYIEKVRFLEQQNKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTTNSARLEVERDNLTQDLGTLRQKLQDETNLRLEAENNLAVYRQEADEATLARVDLERKVESLEEEIQFLRKIHEEEVRELQEQLAQQQVHVEMDVAKPDLTAALREIRTQYEAVATSNMQETEEWYRSKFADLTDVASRNAELLRQAKHEANDYRRQLQALTCDLESLRGTNESLERQMREQEERHARESASYQEALARLEEEGQSLKEEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.功能2:
/文献:
"Identification and characterization of GFAPkappa, a novel glial fibrillary acidic protein isoform." Blechingberg J., Holm I.E., Nielsen K.B., Jensen T.H., Joergensen A.L., Nielsen A.L. Glia 55:497-507(2007)研究领域:
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文献和实验如下: 1.确定细胞类型和形态组织细胞内有些蛋白具有组织特异性,如胶质原纤维酸性蛋白(gial fibrillary acidic protein,GFAP)只存在于星形胶质细胞内.神经丝蛋白(Neurofilament,NF)只存在于神经细胞内。通常把这些具有组织特异性的蛋白称为标记性蛋白(Proteiniliaker)。通过标记性蛋白的特异性抗体可确定细胞种类。有些细胞(如表皮内朗格汉斯细胞和黑色素细胞等)在光镜下不易辨认,通过对胞质内的特定蛋白实施免疫 组化染色,便能清楚显示此类
An In Vitro Trauma Model to Study Rodent and Human Astrocyte Reactivity
opportunity for future studies on glial biology, maturation, and pathology of human astrocytes. Prototypical astrocyte proteins including GFAP, S100, aquaporin4, glutamate transporters, and tenascin are expressed in mouse, rat
Imaging In Vivo Astrocyte/Endothelial Cell Interactions at the BloodBrain Barrier
tight junction protein expression and loss of BBB integrity. As glial fibrillary acidic protein (GFAP)-immunopositive astrocytes repopulated the lesion, tight junction protein expression returned to paracellular domains and BBB
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