GPRC5D/GPRC5D蛋白Recombinant Hum

纯度：

The purity information is not available for VLPs proteins.

基因名：

GPRC5D

Uniprot No.：

Q9NZD1

别名：

(GPRC5D)(G-protein coupled receptor family C group 5 member D)

种属：

Homo sapiens (Human)

蛋白长度：

Full Length

来源：

Mammalian cell

分子量：

40.6 kDa

表达区域：

1-345aa

氨基酸序列：

MYKDCIESTGDYFLLCDAEGPWGIILESLAILGIVVTILLLLAFLFLMRKIQDCSQWNVLPTQLLFLLSVLGLFGLAFAFIIELNQQTAPVRYFLFGVLFALCFSCLLAHASNLVKLVRGCVSFSWTTILCIAIGCSLLQIIIATEYVTLIMTRGMMFVNMTPCQLNVDFVVLLVYVLFLMALTFFVSKATFCGPCENWKQHGRLIFITVLFSIIIWVVWISMLLRGNPQFQRQPQWDDPVVCIALVTNAWVFLLLYIVPELCILYRSCRQECPLQGNACPVTAYQHSFQVENQELSRARDSDGAEEDVALTSYGTPIQPQTVDPTQECFIPQAKLSPQQDAGGV

蛋白标签：

C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)

产品提供形式：

Lyophilized powder

缓冲液：

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

货期：

3-7 business days

注意事项：

The VLPs are expressed from human 293 cells (HEK293).Mix the sample gently by repeatedly pipetting it up and down. Do not vortex.Repeated freezing and thawing is not recommended.Store the protein at -20℃/-80℃ upon receiving it, and ensure to avoid repeated freezing and thawing, otherwise, it will affect the protein activity. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).

功能：

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内毒素：

Less than 1.0 EU/ug as determined by LAL method.

SDS-PAGE:

CSB-MP882153HU is detected by Mouse anti-6*His monoclonal antibody.

LC-MS Image Description/Western Blot:

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Product types:

MP-VLP Transmembrane Protein & Active Protein & In Stock Protein

Biological_Activity:

Measured by its binding ability in a functional ELISA. Immobilized Human GPRC5D at 5 μg/mL can bind Anti-GPRC5D recombinant antibody (CSB-RA882153MA01HU), the EC50 is 8.847-10.38 ng/mL.

Research Areas:

Signal Transduction

Reconstitution:

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein indeionized sterile water to a concentration of 0.1-1.0 mg/mL.Aliquot for long-term storage at -80℃. Solubilize for 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Reference:

"Cloning and characterization of a human orphan family C G-protein coupled receptor GPRC5D." Braeuner-Osborne H., Jensen A.A., Sheppard P.O., Brodin B., Krogsgaard-Larsen P., O'Hara P. Biochim. Biophys. Acta 1518:237-248(2001)

Function:

GPRC5D in MM cells makes this gene and its encoded surface protein as promising markers for monitoring the tumor load and hopefully also as targets for antimyeloma antibodies. PMID: 23510526 Overexpression of G protein-coupled receptor 5D in the bone marrow is associated with poor prognosis in patients with multiple myeloma. PMID: 22591013