Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
浓度:
100μg/瓶
规格:
100μg
GAPDH mouse monoclonal antibody Cat.# Package100 μl StoragePBS with 0.1% sodium azide and 50% glycerol, pH 7.2
Product Imformation
Description Anti-GAPDH mouse monoclonal antibody Applications WB/ IHC/ IF Immunogen Fusion protein of human GAPDH Reactivity Human, Mouse, Rat Host species Mouse Ig class IgG1 Purity Protein A/G Affinity Purification
Target information
SymbolGAPDH Full name glyceraldehyde-3-phosphate dehydrogenase Synonyms G3PD; GAPD; HEL-S-162eP
Target Background
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded proteinhas been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Theproduct of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidativephosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adeninedinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in thenucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans.Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclearproteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Manypseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcriptvariants.
Western blotting
Predicted band size36 kDa Positive control: HUVEC cell and Human left lung tissue lysates Recommended dilution :1000-5000
Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-2: HUVEC cell and Human left lung tissue lysates Primary antibody: GAPDH Antibody at dilution 1/1200 Secondary antibody: Goat anti mouse IgG at 1/5000 dilution Exposure time: 3 minutes