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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
RAMOS
- 库存:
100
- 供应商:
中乔新舟
- 品系:
细胞系
- 运输方式:
常温
- 年限:
液氮长期
- 生长状态:
悬浮
- 规格:
T25
|
产品名称 |
RAMOS 人B淋巴细胞瘤细胞 |
|
货号 |
ZQ1021 |
|
产品介绍 |
Ramos (RA 1) 是一种 B 淋巴细胞系,来源于一名患有伯基特 Burkitt 淋巴瘤的 3 岁男孩的腹水中建立的,是免疫学研究的重要资源。该细胞系以分泌 IgM 为特征,对于 B 细胞表面抗原分析、细胞毒性药物检测、突变分析和凋亡机制探索具有重要价值。
特别注意:该细胞为悬浮细胞,请注意离心收集细胞悬液。细胞形态有部分悬浮聚小团细胞是正常现象 |
|
种属 |
白人 |
|
性别/年龄 |
男/3岁 |
|
组织 |
淋巴 |
|
疾病 |
伯基茨淋巴瘤 |
|
细胞类型 |
肿瘤细胞 |
|
形态学 |
圆形 |
|
生长方式 |
悬浮 |
|
倍增时间 |
20.4 +- 1.5 hours (PubMed=9225077); 23 hours (PubMed=8402660); 14 hours (PubMed=20922763); ~48 hours (DSMZ=ACC-603) |
|
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
|
推荐完全培养基货号 |
ZM1021 |
|
生物安全等级 |
BSL-1 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
|
STR位点信息 |
Amelogenin: X CSF1PO :10,11 D1S1656 :12,15.3 D2S441: 11,14 D2S1338: 20,23 D3S1358: 14,15 D5S818: 7,12 D6S1043 :13,15 D7S820 :11 D8S1179: 13 (CLS=302007) :13,16 (ATCC=CRL-1596; CCRID; DepMap=ACH-001636; DSMZ=ACC-603; Technion Genomics Center BCF; PubMed=20922763; PubMed=25877200) D10S1248: 14,15 D12S391: 19,22 D13S317: 12,13,14 (CLS=302007) :13,14 (ATCC=CRL-1596; CCRID; COG; DepMap=ACH-001636; DSMZ=ACC-603; ECACC=85030802; Technion Genomics Center BCF; JCRB=JCRB9119; KCLB=21596; PubMed=20922763; PubMed=25877200) D16S539 :10,13 D18S51: 14,15 (CLS=302007; DSMZ=ACC-603) :15 (ATCC=CRL-1596; CCRID; DepMap=ACH-001636; Technion Genomics Center BCF; PubMed=20922763; PubMed=25877200) D19S433: 14,15.2 D21S11: 30 D22S1045: 15 FGA: 20,24 Penta D :10,13 Penta E: 6,8,21 (CLS=302007) :8,21 (ATCC=CRL-1596; CCRID; DepMap=ACH-001636; DSMZ=ACC- 603; Technion Genomics Center BCF; PubMed=25877200) TH01: 7,9.3 TPOX :8,9 vWA :15,16 |
|
抗原表达/受体表达 |
*** |
|
基因表达 |
*** |
|
保藏机构 |
ATCC; CRL-1596 |
|
供应限制 |
仅供科研使用 |
上海中乔新舟生物科技有限公司成立于2011年,历经十多年发展,主要专注于细胞生物学产品的研究和开发,专注于为药企、各类科研机构及CRO企业提供符合标准规范的细胞培养服务、细胞培养基、细胞检测试剂盒、细胞培养试剂,胎牛血清和细胞生物学技术服务等。
公司一直致力于为高等院校、研究机构、医院、CRO及CDMO企业提供细胞培养完整解决方案,这些产品旨在满足细胞培养的多样需求,确保实验和研究的有效进行。引用中乔新舟(ZQXZBIO)产品和服务的文献超数千篇。

产品服务
细胞资源:原代细胞、细胞株、干细胞、示踪细胞、耐药株细胞、永生化细胞等基因工程细胞。
试剂产品:胎牛血清、完全培养基(适用于原代细胞及细胞株)、无血清培养基、基础培养基、细胞转染试剂、重组因子、胰酶和双抗等等细胞培养所有实验相关产品。
技术服务:稳转株构建、原代细胞分离、特殊培养基定制服务、细胞检测等。

目前产品已经畅销国内30多个省市,与客户建立长期的合作伙伴关系,共同实现成功。全体员工将不懈努力,继续为科研人员提供优良的产品和服务,致力成为全球细胞培养领域的参与者。

企业愿景
致力于成为国内细胞培养基产业的佼佼者,生物医药领域上游原材料的优良提供商。
企业使命
成长为专业细胞系及原代细胞培养供应商、专业细胞培养基及培养试剂生产商。
企业荣誉


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文献和实验
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An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection.
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Inducibility of the Epstein-Barr virus (EBV) cycle and surface marker properties of EBV-negative lymphoma lines and their in vitro EBV-converted sublines.
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Establishment of EBNA-expressing cell lines by infection of Epstein-Barr virus (EBV)-genome-negative human lymphoma cells with different EBV strains.
Int. J. Cancer 17:161-166(1976)
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Povey S., Jeremiah S., Arthur E., Steel M., Klein G.
Differences in genetic stability between human cell lines from patients with and without lymphoreticular malignancy.
Ann. Hum. Genet. 44:119-133(1980)
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Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type.
J. Immunol. 129:1336-1342(1982)
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Ehlin-Henriksson B., Klein G.
Distinction between Burkitt lymphoma subgroups by monoclonal antibodies: relationships between antigen expression and type of chromosomal translocation.
Int. J. Cancer 33:459-463(1984)
PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.
Leuk. Res. 9:209-229(1985)
PubMed=2998993
Steel C.M., Morten J.E.N., Foster E.
The cytogenetics of human B lymphoid malignancy: studies in Burkitt's lymphoma and Epstein-Barr virus-transformed lymphoblastoid cell lines.
IARC Sci. Publ. 60:265-292(1985)
PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- III Beta-hexosaminidase (E.C. 3.2.1.30).
Leuk. Res. 9:549-559(1985)
PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)
PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)
PubMed=2835030; DOI=10.1016/S0385-8146(87)80025-1
Takimoto T., Sato H., Ogura H., Miyazaki T.
Establishment of an Epstein-Barr virus (EBV) genome-positive subline of Ramos (Ramos/NPC) following infection of Ramos with nasopharyngeal carcinoma (NPC)-derived EBV.
Auris Nasus Larynx 14:87-92(1987)
PubMed=3026973; DOI=10.1002/ijc.2910390215
Ehlin-Henriksson B., Manneborg-Sandlund A., Klein G.
Expression of B-cell-specific markers in different Burkitt lymphoma subgroups.
Int. J. Cancer 39:211-218(1987)
PubMed=1850347; DOI=10.1210/endo-128-5-2266
Baglia L.A., Cruz D., Shaw J.E.
An Epstein-Barr virus-negative Burkitt lymphoma cell line (sfRamos) secretes a prolactin-like protein during continuous growth in serum-free medium.
Endocrinology 128:2266-2272(1991)
PubMed=1915267; DOI=10.1002/j.1460-2075.1991.tb07837.x
Farrell P.J., Allan G.J., Shanahan F., Vousden K.H., Crook T.
p53 is frequently mutated in Burkitt's lymphoma cell lines.
EMBO J. 10:2879-2887(1991)
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p53 mutations in human lymphoid malignancies: association with Burkitt lymphoma and chronic lymphocytic leukemia.
Proc. Natl. Acad. Sci. U.S.A. 88:5413-5417(1991)
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Evans H.H., Ricanati M., Horng M.-F., Jiang Q.-Y., Mencl J., Olive P.L.
DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.
Radiat. Res. 134:307-315(1993)
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FASEB J. 7:951-956(1993)
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Role of the p53 tumor suppressor gene in cell cycle arrest and radiosensitivity of Burkitt's lymphoma cell lines.
Cancer Res. 53:4776-4780(1993)
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Variable IgH chain enhancer activity in Burkitt's lymphomas suggests an additional, direct mechanism of c-myc deregulation.
J. Immunol. 150:5418-5428(1993)
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Cancer Res. 55:2387-2393(1995)
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Blood 88:3562-3568(1996)
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亦可简称B细胞。来源于骨髓的多能干细胞。在禽类是在法氏囊内发育生成,故又称囊依赖淋巴细胞(bursa dependent lymphocyte)。在哺乳类是在类囊结构的骨髓等组织中发育的。又称骨髓依赖淋巴细胞。从骨髓来的干细胞或前B细胞,在迁入法氏囊或类囊器官后,逐步分化为有免疫潜能的B细胞。成熟的B细胞经外周血迁出,进入脾脏、淋巴结,主要分布于脾小结、脾索及淋巴小结、淋巴索及消化道粘膜下的淋巴小结中,受抗原刺激后,分化增殖为浆细胞,合成抗体,发挥体液免疫的功能。B细胞
处理的瘤细胞作为肿瘤疫苗 肿瘤疫苗包括灭活的自体肿瘤细胞、提取的肿瘤抗原和人工合成的肿瘤抗原肽,通过给患者免疫接种,激发患者自身对肿瘤细胞的特异性免疫应答,清除肿瘤而不损伤周围正常细胞。肿瘤疫苗还可诱发免疫记忆细胞,产生长期的免疫效应,防止肿瘤的转移和复发。是一种理想的特异性主动免疫治疗手段。 肿瘤疫苗激活T细胞作用机制 肿瘤细胞表达的肿瘤抗原经APC加工处理,通过MHCI类和Ⅱ类分子提呈至细胞表面,分别激活CD
人前列腺癌细胞 RAJI 黑人Burkitt淋巴瘤 RAMOS 人B淋巴细胞瘤 RAMOS(RA.1) 人B淋巴细胞瘤 RPMI-8226 人多发骨髓瘤细胞 SaOS-2 人骨肉瘤细胞 SF126 人脑瘤 SF17 人脑瘤 SF17 人脑瘤 SF763 人脑瘤 SF767 人脑瘤 SH-SY5Y 人骨髓神经母细胞瘤 SK-BR-3 人乳腺癌细胞 SK-HEL-1 人皮肤黑色素瘤细胞 SK-N-SH 人神经母细胞瘤 SK-OV-3 人卵巢
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